| Influence of rotation fallow mode on bacterial community in soil |
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| DOI：10.7606/j.issn.1000-7601.2020.06.18 |
| Key Words: fallow rotation mode soil bacterial community abundance soil nutrient semi\|arid region of Loess Plateau |
| Author Name | Affiliation | | NAN Lili | College of Pratacultural Science, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | GUO Quanen | Soil Fertilizer and Water-Saving Institute, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu 730070, China | | TAN Jiehui | College of Pratacultural Science, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | KANG Fayun | The Agricultural Technique Popularization of Yongjing county in Gansu Province, Yongjing, Gansu 731600, China |
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| Abstract: |
| Soil bacterial community composition, abundance, and diversity were studied in nine rotation fallow modes in semi\|arid region of Loess Plateau. Nine rotation fallow treatments as: a control (CK) with continuous three years of fallow; a three\|year residual plastic film mulching (T1); a three\|year deep tillage in hot summer (T2); applying cattle and sheep manure of 7 500 kg·hm-2, then deep tillage of 30 cm for 3 consecutive years of fallow (T3); applying cattle and sheep manure of 7 500 kg·hm-2, after deep tillage of 30 cm planting Pisum sativum for 3 consecutive years and returning to the field in full bloom (T4); applying cattle and sheep manure of 7 500 kg·hm-2, after deep tillage of 30 cm planting Pisum sativum with rhizobium powder 8.5 g·kg-1 for 3 consecutive years and returning to the field in full bloom (T5); corn straw chopping and returning to the field of 7 500 kg·hm-2, then deep tillage of 30 cm for 3 consecutive years (T6); corn straw chopping and returning to the field of 7 500 kg·hm-2, after deep tillage of 30 cm planting Iicia villosa and returning to the field in full bloom (T7); and corn straw chopping and returning to the field of 7 500 kg·hm-2, then applying cattle and sheep manure of 7 500 kg·hm-2, after deep tillage of 30 cm planting Vicia sativa and returning to the field in full bloom (T8). The determination of the soil bacterial community characteristics was achieved by DNA extraction followed by PCR amplification, and sequencing using the HiSeq platform. The 16 SrRNA amplifier sequencing technique was used in the study. The correlation between soil nutrients and bacterial population diversity was analyzed. The results showed that the top five phyla with highest relative abundance were Proteobacteria (26.33%~37.33%), Acidobacteria (17.93%~21.43%), Actinobacteria (12.42%~19.41%), Gemmatimonadetes (6.98%~10.99%), and Chloroflexi (6.48%~10.50%) under the nine rotation fallow modes. The relative abundance of Nitrospirae in T1 and T5 treatments significantly improved by 50.04 and 42.44%, respectively, the relative abundances of Proteobacteria and Bacteroidetes in T2 treatment significantly increased by 31.51% and 159.96% respectively, the relative abundances of Gemmatimonadetes in T6 and T7 treatments significantly increased by 28.36% and 28.33%, respectively, the relative abundance of Thaumarchaeota in T8 treatment significantly increased by 519.79%. Soil total nitrogen content of all treatments was significantly higher than that of CK. The abundance indexes (ACE and Chao1) of all treatments except T7 treatment were significantly higher than that of CK. Redundancy analysis (RDA) showed that the compositions of the soil bacterial communities were driven by several soil chemical proprieties. The Monte Carlo permutation test showed that the soil total nitrogen, total potassium, and available nitrogen contents significantly influenced the composition of the bacterial community. |
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