| Functional characterization of a chromatin remodeling factor Vmles4 in Valsa mali |
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| DOI：10.7606/j.issn.1000-7601.2020.06.32 |
| Key Words: Valsa mali INO80 subunits Vmles4 knockout mutants growth rate pathogenicity |
| Author Name | Affiliation | | DU Hongxia | College of Plant Protection, State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100, China | | NIE Jiajun | College of Plant Protection, State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100, China | | FENG Yaqiong | College of Plant Protection, State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100, China | | LIU Jianying | College of Plant Protection, State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100, China | | HUANG Lili | College of Plant Protection, State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100, China |
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| Abstract: |
| Chromatin remodeling factor INO80 is a kind of genetic regulatory factors composed of multiple subunits that regulate multiple DNA metabolism and play an important regulatory role in gene expression. But, it is not clear whether it exists in Valsa mali and its biological functions. Therefore, a subunit gene Vmles4 of INO80 was analyzed from the fungal genome in this study. The expression patterns of Vmles4 was analyzed by qRT-PCR technology during the infection process. Using Double\|joint PCR techniques and PEG mediated protoplast transformation methods for gene knock out. Then, the vegetative growth and pathogenicity of 3 mutants were measured and analyzed. The expressions of non\|ribosomal peptide synthase genes VmNRPS12 and VmNRPS14 in mutants were analyzed quantitatively. The results showed that the expression of Vmles4 was up\|regulated significantly in the early stage of infection, and 6.2 times in 6h after inoculation. Growth rate of knockout mutants decreased by 28% on average and mycelium growth was sparse, and the pathogenicity in leaves and twigs of Fuji apple varieties (Malus domestic cv.Fuji) decreased to 22.5% and 27.5%, respectively. The expression of VmNRPS12 and VmNRPS14 genes was down\|regulated by 86.5% and 50%, respectively after 24 h of infection of twigs by Vmles4 mutant. In conclusion, Vmles4 is regulating the vegetative growth, pathogenicity, and the expression of secondary metabolic synthase gene VmNRPS12 and VmNRPS14 of Valsa mali. |
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