| Fatty acid components and differential expression of FAD3 in winter Brassica rapa L. under low temperature stress |
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| DOI：10.7606/j.issn.1000-7601.2021.01.09 |
| Key Words: FAD3 winter Brassica rapa L. cold stress fatty acid IUFA differential expression |
| Author Name | Affiliation | | SUN Bolin | College of Agronomy, Gansu Agricultural University / Rapeseed Engineering Research Center of Gansu Province/Key Laboratory of Arid Land Crop Science in Gansu Province / Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou , Gansu 730070, China | | MA Li | College of Agronomy, Gansu Agricultural University / Rapeseed Engineering Research Center of Gansu Province/Key Laboratory of Arid Land Crop Science in Gansu Province / Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou , Gansu 730070, China | | ZENG Xiucun | School of Life Sciences and Engineering Hexi University, Zhangye, Gansu 734000 China | | NIU Zaoxia | College of Agronomy, Gansu Agricultural University / Rapeseed Engineering Research Center of Gansu Province/Key Laboratory of Arid Land Crop Science in Gansu Province / Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou , Gansu 730070, China | | WANG Wanpeng | College of Agronomy, Gansu Agricultural University / Rapeseed Engineering Research Center of Gansu Province/Key Laboratory of Arid Land Crop Science in Gansu Province / Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou , Gansu 730070, China | | HU Fangdi | College of Agronomy, Gansu Agricultural University / Rapeseed Engineering Research Center of Gansu Province/Key Laboratory of Arid Land Crop Science in Gansu Province / Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou , Gansu 730070, China | | LU Xiaoming | College of Agronomy, Gansu Agricultural University / Rapeseed Engineering Research Center of Gansu Province/Key Laboratory of Arid Land Crop Science in Gansu Province / Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou , Gansu 730070, China | | QI Weiliang | College of Agronomy, Gansu Agricultural University / Rapeseed Engineering Research Center of Gansu Province/Key Laboratory of Arid Land Crop Science in Gansu Province / Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou , Gansu 730070, China | | WU Junyan | College of Agronomy, Gansu Agricultural University / Rapeseed Engineering Research Center of Gansu Province/Key Laboratory of Arid Land Crop Science in Gansu Province / Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou , Gansu 730070, China | | LIU Lijun | College of Agronomy, Gansu Agricultural University / Rapeseed Engineering Research Center of Gansu Province/Key Laboratory of Arid Land Crop Science in Gansu Province / Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou , Gansu 730070, China | | LI Xuecai | College of Agronomy, Gansu Agricultural University / Rapeseed Engineering Research Center of Gansu Province/Key Laboratory of Arid Land Crop Science in Gansu Province / Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou , Gansu 730070, China | | SUN Wancang | College of Agronomy, Gansu Agricultural University / Rapeseed Engineering Research Center of Gansu Province/Key Laboratory of Arid Land Crop Science in Gansu Province / Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou , Gansu 730070, China | | MIAO Chunqing | Zhangye Academy of Agricultural Sciences, Zhangye, Gansu 734000 China | | WANG Xuefang | Gansu Agricultural Technology College, Lanzhou, Gansu 730070, China | | LI Xiaoze | Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, Gansu 730070, China | | FANG Yan | College of Agronomy, Gansu Agricultural University / Rapeseed Engineering Research Center of Gansu Province/Key Laboratory of Arid Land Crop Science in Gansu Province / Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou , Gansu 730070, China |
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| Abstract: |
| In order to explore the response of fatty acid composition to low temperature stress and the cold resistance mechanism of FAD3 (ω-3-Fatty acid desaturase) in winter Brassica rapa L., The changes of fatty acid composition in roots and leaves of three different cold resistant types of winter rapeseed (Brassica campestris L.) under low temperature stress were determined by gas chromatography Mass Spectrometry (GC-MS). The CDS sequence of omega-3 fatty acid desaturase (FAD3) of Longyou 7 was obtained by PCR technique. The FAD3 protein sequence of Longyou 7 was analyzed by bioinformatics. The results showed that under low temperature stress, the contents of linoleic acid and linolenic acid in roots of strong cold\|resistant variety Longyou 7 increased by 157% and 89%, respectively, compared with non\|cold\|resistant variety Lenox, and the value of IUFA in root of Longyou 7 increased by 91% compared with Lenox. That is, under low temperature stress, linoleic acid, linolenic acid, and IUFA of cold\|resistant varieties were significantly higher than those of non\|cold\|resistant varieties. The results showed that the CDS region of Longyou 7 FAD3 sequence encodes 429 amino acids with a molecular weight of 49.16 kD and an isoelectric point of 8.60. It is a stable hydrophilic protein. Multiple sequence alignment analysis showed that its encoded protein had the highest similarity with Brassica rapa in the same family, reaching 100%. The result of evolutionary tree analysis also showed that the relationship between FAD3 protein and Brassica rapa was closely correlated. Fluorescence quantitative expression analysis showed that under low temperature stress, the expression of FAD3 in Longyou 7, Tianyou 2, and Lenox roots increased by 690%, 590%, and 710%, respectively, compared with the control (22℃). The results showed that FAD3 was up\|regulated to adapt to low temperature, and its expression was positively correlated with fatty acid unsaturation index (IUFA). The above studies provided ideas for understanding the function of [STBX]FAD3 protein and the composition of fatty acids in winter Brassica rapa L., exploring its role in stress resistance and cultivating stress\|resistant germplasm resources. |
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