| Cloning of soybean DNA demethylase related genes and response to continuous cropping stress |
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| DOI：10.7606/j.issn.1000-7601.2021.01.11 |
| Key Words: DNA demethylase gene cloning gene expression continuous cropping comprehensive stress response to continuous cropping stress soybean |
| Author Name | Affiliation | | FANG Shumei | Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | HOU Xue | Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | QIU Caowei | Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | HAN Bei | Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | HU Junjie | Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | LIANG Xilong | Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China |
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| Abstract: |
| The soybean genome database phytozome v12.1.6 was used to obtain the CDS sequences of soybean DNA demethylase\|related genes Glyma03g34860 and Glyma10g07601. Using soybean leaf RNA as template, 5 226 bp Glyma03g34860 and 6 045 bp Glyma10g07601 were successfully cloned by RT-PCR method. The interacting proteins predicted by using STRING online software were completely the same. There were 8 main interaction proteins that include one AP site lyase and two RNA polymerase subunits. The qRT-PCR results showed that the expressions of Glyma03g34860 and Glyma10g07601 were both up\|regulated with different degree under continuous cropping stress. Of these, the level of Glyma10g07601 was significantly increased by 1.37, 1.22, 1.98 times, and 1.67 times in Andanongjia, Heidadou, Suinong 14, and heinong 40 varieties,respectively (P<0.05), while the level of Glyma03g34860 was significantly increased by 1.62 times and 1.65 times in Kenfeng 16 and Suinong 14 varieties, respectively (P<0.05), suggesting that they participated in the response on continuous cropping comprehensive stress through demethylating genomic DNA in soybean. |
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