| The molecular evolution of maize DUF581 gene family and expression patterns in response to drought stress |
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| DOI：10.7606/j.issn.1000-7601.2021.01.12 |
| Key Words: maize drought stress DUF581 molecular evolution transcriptional expressional profiles |
| Author Name | Affiliation | | LI Zelong | College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China | | ZHU Ying | College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China | | ZHANG Chengyu | College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China | | GUO Yingbin | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100， China | | HAN Zhaoxue | College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China |
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| Abstract: |
| The 45 members of DUF581 gene family in maize were identified using bioinformatics methods in this study, and the sequence analysis of the DUF581 genes showed the physiochemical properties of the gene family were conserved among different species. Based on the phylogenetic tree, the family genes were divided into six clades, CladeⅠ~Ⅵ. The structure of the maize DUF581 gene in clades II~Ⅵ was similar and conserved, and these members contained 1 to 2 exons; while the number of exons of family members in clade I contained five on average. Maize DUF581 genes were distributed on 10 chromosomes. Gene duplication events analysis showed that 14 tandem duplication genes in maize were located in 4 tandem duplication gene groups. Two segmental duplication gene pairs Zm00001d002530/Zm00001d017646 and Zm00001d017646/Zm00001d051496 were also tandem duplications. Expression pattern analysis of maize DUF581 genes in three tissues (leaf, ear, and tassel) at four developmental stages (V12, V14, V18, and R1) under normal growth conditions demonstrated that nearly half (22/45) of the maize DUF581 gene were ubiquitously expressed in three tissues at four stages, and had higher expression levels in the two reproductive tissues of ear and tassel. A total of 26 differentially expressed genes (DEGs) were identified under drought stress. All the DEGs in ear at V14 and V18 stages, and tassel at V12 and V14 stages were down\|regulated, while most of the DEGs in ear at V12 and R1 stages were up\|regulated, and more, most of the duplication pairs showed different expression patterns. |
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