| ]Establishment of buckwheat drought tolerance identification system at germination stage and screening of drought\|tolerance germplasms |
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| DOI：10.7606/j.issn.1000-7601.2021.01.13 |
| Key Words: drought tolerance indentification system drought tolerance indices, drought tolerance identification common buckwheat germination period |
| Author Name | Affiliation | | HOU Zehao | College of Agriculture, Yangtze University/ Hubei Center for Collaborative Innovation of Grain Industry, Jingzhou, Hubei 434025, China | | LU Yifei | College of Agriculture, Yangtze University/ Hubei Center for Collaborative Innovation of Grain Industry, Jingzhou, Hubei 434025, China | | SUN Kunkun | College of Agriculture, Yangtze University/ Hubei Center for Collaborative Innovation of Grain Industry, Jingzhou, Hubei 434025, China | | WANG Shuping | College of Agriculture, Yangtze University/ Hubei Center for Collaborative Innovation of Grain Industry, Jingzhou, Hubei 434025, China | | ZHANG Yingxin | College of Agriculture, Yangtze University/ Hubei Center for Collaborative Innovation of Grain Industry, Jingzhou, Hubei 434025, China | | LIU Zhixiong | College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei 434025, China | | FANG Zhengwu | College of Agriculture, Yangtze University/ Hubei Center for Collaborative Innovation of Grain Industry, Jingzhou, Hubei 434025, China |
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| Abstract: |
| In order to explore the suitable stress concentration and index for the identification of the drought tolerance of common buckwheat and establish a buckwheat drought tolerance identification system at germination stage, and screening of drought\|tolerance common buckwheat germplasm resources, varying concentration of polyethylene glycol (PEG) 6000 (10%, 20%, 25%, and 30%) were used for analysis of the effects of different drought stress levels on the seed germination and growth of common buckwheat. Eighty common buckwheat accessions from different regions were subjected for drought tolerance tests upon drought stress treatment. The results showed that the low concentration stress (10% PEG6000) lightly affected the seed germination and the growth of root, and the sprout index of drought resisting (SIDR) ranged from 80.92% to 109.31%. The relative germination rate, SIDR, plant height, and root length were significantly reduced with increasing concentration of PEG6000. Under the stress of 20% PEG6000 solution, the SIDR was between 50.82% and 101.62%, and under the stress of 25% PEG6000 and 30% PEG6000 solution, the SIDR of all varieties was below 50% and 5%, respectively, which significantly inhibited the seed germination. Therefore, 20% PEG6000 was considered as the optimal concentration for common buckwheat drought tolerance identification. The significant correlations on drought tolerant coefficient were observed between sprout index of drought resisting, relative germination rate, relative root length index, and root length stress index. These four indices were recommended for drought resistance evaluation in common buckwheat. The 80 common buckwheat germplasm materials were classified into four groups, composing of 3 germplasm materials with high tolerance (3.75%), 13 with tolerance (16.25%), 33 with moderate tolerance (41.25%), and 31 with susceptibility (38.75%). |
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