| Deep sowing alleviating effect of exogenous Gibberellin on maize inbred lines with different depth sowing tolerance |
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| DOI：10.7606/j.issn.1000-7601.2021.02.06 |
| Key Words: maize deep sowing stress exogenous Gibberellin alleviating effect |
| Author Name | Affiliation | | LU Yantian | College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | CHEN Fenqi | College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | BAI Mingxing | College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | ZHUANG Zelong | College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | DING Yongfu | College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | PENG Yunling | Gansu Provincial Key Lab of Aridland Crop Science, Gansu Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou, Gansu 730070, China |
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| Abstract: |
| To explore the different concentrations of exogenous Gibberellin (GA) on the germination and seedling traits of maize seeds under deep sowing stress, the mitigation effect of GA on deep sowing was comprehensively evaluated. In this experiment, two deep-seeding sensitive inbred lines and two deep-seeding resistant inbred lines were used as test materials. At seedling stage, four exogenous GA with different concentrations were added under two kinds of deep-seeding stress. The results showed that: (1) The seeding emergence rate, seedling length and root length were significantly reduced under deep sowing stress, the length of mesocotyl and coleoptile, the sum length of mesocotyl and coleoptile, SOD activity in coleoptile, POD, CAT activity, and lignin content in mesocotyl and coleoptile increased significantly. The RC was between -75.86%~100.19%. After the addition of exogenous GA, all seedling traits increased significantly by 4.25%~389.06%, respectively. (2) Screen the optimal concentration of exogenous GA with two deep-seeding sensitive inbred lines, the relief effect of applying 4.0 mg·L-1 and 6.0 mg·L-1 exogenous GA under 15 cm and 20 cm deep sowing stress were optimal. The membership function values were 0.602 and 0.598. (3) At the optimal concentration, comparative analysis of the growth parameters and physiological characteristics of two maize inbred lines with different sowing tolerances showed that exogenous GA alleviated the stress damage of maize seedlings by deep sowing and restore physiological and biochemical metabolic steady state. However, the exogenous GA had different sensitivity to maize inbred lines with different tolerance to deep sowing, Ji853 > Zi330 > Mo17 > N192. (4) In order to further accurately evaluate the tolerance of different varieties of maize to deep sowing, the main components and heat map analysis were used to obtain the three-level evaluation indicators for the identification of deep sowing at the germination stage of maize: emergence rate, mesocotyl axis length, mesocotyl axis, and the sum of sheaths was the main evaluation index. The SOD， POD， CAT activity in the mesocotyl, and the lignin content in the mesocotyl and coleoptile can be used as secondary evaluation indicators, and the number of roots and seedling length can be used as third-level evaluation indicators. |
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