| Effects of exogenous ABA and sodium tungstate on ZEP expression in winter Brassica rapa under drought stress |
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| DOI：10.7606/j.issn.1000-7601.2021.02.07 |
| Key Words: winter Brassica rapa drought stress exogenous ABA exogenous sodium tungstate ZEP gene clone expression |
| Author Name | Affiliation | | LI Xuecai | Gansu Provincial Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | CHANG Yu | Gansu Provincial Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | BAI Jing | Gansu Provincial Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | WU Junyan | Gansu Provincial Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | LI Bowen | Gansu Provincial Agro-Technology Extension Station, Lanzhou, Gansu 730020, China | | ZHAO Yuhong | Gansu Provincial Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | SUN Bailin | Gansu Provincial Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | WANG Wanpeng | Gansu Provincial Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | TANG Deyao | Kang County Tea Science and Technology Development Center, Longnan, Gansu 746500, China | | FAN Xiaolong | Kongtong District Agro-technology Extension Center, Pingliang, Gansu 744000, China | | ZHU Wenying | Kongtong District Agro-technology Extension Center, Pingliang, Gansu 744000, China | | MA Li | Gansu Provincial Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | SUN Wancang | Gansu Provincial Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China |
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| Abstract: |
| In order to study the molecular mechanism of zeaxanthin epoxidase (ZEP) gene in response to exogenous ABA under drought stress, in this study, winter rapeseed Longyou7 and Tianyou4 were selected as test materials, and 200 mL of distilled water, ABA solution, sodium tungstate solution, and ABA + sodium tungstate were sprayed, respectively, and placed in a plant incubator for 7 days for drought stress. RT-PCR and qPCR were used to clone and analyze the expression of ZEP gene, the bioinformatics analysis showed that the gene contained an ORF of 2013 bp, encoding a protein with 670 amino acid residues, and the theoretical isoelectric point was 6.16, belonging to hydrophilic protein. The homology of the gene with Arabidopsis thaliana and Brassica napus was 89.25% and 99.40%, respectively. The real-time fluorescence quantitative analysis showed that drought stress could induce the up regulation of ZEP gene expression in winter rapeseed. The relative expression of ZEP gene in leaves of Longyou7 increased by 66.64% compared with the CK after spraying exogenous ABA, and the expression of ZEP gene in leaves of Longyou7 decreased by 12.74% compared with the CK after spraying exogenous ABA synthesis inhibitor sodium tungstate. After spraying exogenous ABA and sodium tungstate at the same time, the expression of ZEP gene in leaves of Longyou7 and Tianyou4 increased by 3.3 and 2.28 times, respectively, compared with the CK under drought stress, and the expression of ZEP gene in leaves of Longyou 7 was 21.61% higher than that in roots, indicating that ZEP gene may play a role in drought resistance of winter Brassica rapa. |
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