| Effects of exogenous hydrogen sulfide on stress resistance physiological characteristics of cotton seedlings under NaCl stress |
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| DOI：10.7606/j.issn.1000-7601.2021.02.08 |
| Key Words: exogenous hydrogen sulfide NaCl stress cotton seedlings photosynthetic characteristic activity of antioxidant enzymes |
| Author Name | Affiliation | | CHEN Mingyuan | College of Agriculture, Shihezi University, Shihez, Xinjiang 832000, China | | ZHANG Shuying | College of Agriculture, Shihezi University, Shihez, Xinjiang 832000, China | | WANG Mengke | College of Agriculture, Shihezi University, Shihez, Xinjiang 832000, China | | WU Ying | College of Agriculture, Shihezi University, Shihez, Xinjiang 832000, China |
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| Abstract: |
| In order to investigate the regulatory mechanism of exogenous hydrogen sulfide (H2S) on physiological response of cotton seedlings under salt stress, Xinluzao 48 was used as a cotton variety for testing material. The experiment was conducted by a pot culture that roseite was used as the culture pot with nutrient solution. Salt (NaCl, 0, 100 mmol·L-1 and 200 mmol·L-1) and sodium hydrogen sulfide (NaHS, 0, 20, 50, 100 μmol·L-1 and 500 μmol·L-1) were composed to a total of 11 treatments by random combination. The effects of exogenous H2S on the growth, chlorophyll content, chlorophyll fluorescence, photosynthetic gas parameters, and antioxidant enzyme activity of cotton seedlings under salt stress were studied. The results showed that the biomass of cotton seedlings increased in different degrees under the same NaCl and different H2S concentrations, such as T2, T3, T4, and T5 comparing with T1, the above-ground fresh weight of cotton seedlings increased by 35.34%， 28.79%， 9.31%, and 19.66%, respectively. After salt treatment, chlorophyll fluorescence and photosynthetic gas parameters were reduced. When NaCl concentration was 200 mmol·L-1, qP, qN, Y, Y(NPQ), and Y(NO) decreased by 3.52%, 6.14%, 17.06%, and 17.65%, respectively. Net photosynthetic rate, stomatal conductance, intercellular carbon dioxide concentration, and transpiration rate decreased by 18.87%, 49.43%, 15.1%, and 18.81%, respectively. When the concentration of NaCl was 100 mmol·L-1, compared with T1, the content of chlorophyll a, chlorophyll b, and total chlorophyll in T4 increased by 13.04%, 28.09%, and 10%, respectively. When the concentration of NaCl was 200 mmol·L-1, compared with K1, the content of chlorophyll a, chlorophyll b, and total chlorophyll in K4 increased by 14.18%, 52.05%, and 15.23%, respectively. With the increase of salt concentration, the activities of antioxidant enzyme in cotton seedlings were inhibited, and the inhibition effect was alleviated after the application of H2S. For example, compared with K1, the activities of POD, SOD, and CAT in K4 were increased by 85.02%, 28.13%, and 40.47%, respectively. The optimum concentration of H2S was 100 μmol·L-1. |
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