| Evaluation on drought resistance of 187 maize inbred lines and association analysis with SSR markers |
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| DOI：10.7606/j.issn.1000-7601.2021.03.01 |
| Key Words: maize inbred line drought agronomic trait SSR marker association analysis |
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| Abstract: |
| The agronomic traits, namely plant height (PH), ear height (EH), ear height\|to\|plant height ratio (EHPH), tassel branch number (TBN), anthesis\|silking interval (ASI), and ear number per plant (EN) were closely associated with drought resistance in maize (Zea mays L), elucidating the genetic basis of these agronomic traits under different watering environments can provide theoretical support for molecular breeding with drought resistance in maize. Six agronomic traits in 187 maize inbred lines were measured under different watering environments, and the drought resistance of corresponding inbred lines were comprehensively evaluated; and then the association between molecular markers and six agronomic traits under different watering environments were detected with 145 SSR markers in this study. The results showed that: (1) The ASI significantly increased in 187 inbred lines under drought stress, however, other five agronomic traits significantly decreased, and the degrees of change on these traits under drought stress were -72.73%~11.96%. (2) The drought resistance coefficient of the above six traits were used as the evaluation index of drought resistance in maize, and then 45 strong drought resistant inbred lines, 74 medium drought resistant inbred lines, and 68 drought sensitive inbred lines were screened out by the membership function method. (3) Total 13 SSR markers were found to associated with (P<0.01) these six traits under different watering environments by general linear model (GLM) program, the corresponding significant SSR were distributed on chromosomes 1, 2, 3, 4, 5, 7, 8, and 9, and the phenotypic variance explained were 2.15%~17.13%, then approximately 85.19% SSR were detected under drought stress; The bnlg149 (Bin 1.01), phi053 (Bin 3.05), umc1621a (Bin 4.06), umc1155 (Bin 5.05), umc1072 (Bin 5.07), and umc1120 (Bin 9.04) were simultaneous associated with multiple agronomic traits under different watering environments, which significantly showed “a pleiotropic effect”. These results can provide useful information for selection and improvement of drought resistant inbred lines, and marker\|assisted selection (MAS) breeding with drought resistance in maize. |
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