| Assessment of drought tolerance of rapeseed seedling and the evaluation of drought indexes |
|
View Fulltext View/Add Comment Download reader |
| |
| DOI：10.7606/j.issn.1000-7601.2021.03.02 |
| Key Words: Brassica napus L. drought stress comprehensive evaluation seedling stage survival rate drought resistance index |
| Author Name | Affiliation | | HUANG Qian | Institute of Crop Science/Ministry of Agriculture Key Lab of Spectroscopy Sensing， Zhejiang University, Hangzhou, Zhejiang 310058, China | | ZHAO Yongguo | Guangdong University of Petrochemical Technology, Maoming, Guangdong 525000, China | | XU Jinsong | Yangtz University/Engineering Research Center of Ecology and Agricultural Use of Wetland Ministry of Education, Jingzhou, Hubei 434023, China | | ZENG Liu | Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, Hubei 430062, China | | LV Yan | Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, Hubei 430062, China | | CHENG Yong | Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, Hubei 430062, China | | ZHANG Xuekun | Yangtz University/Engineering Research Center of Ecology and Agricultural Use of Wetland Ministry of Education, Jingzhou, Hubei 434023, China | | LU Guangyuan | Guangdong University of Petrochemical Technology, Maoming, Guangdong 525000, China |
|
| Hits: 865 |
| Download times: 208 |
| Abstract: |
| Drought stress is a threat for rapeseed production in China, which necessitates screening and planting of drought tolerant rapeseed varieties. To improve the screening efficiency of drought tolerant rapeseed genotype at seedling stage, we conducted experiments using a representative collection of 79 rapeseed accessions. Rapeseed seedlings were subjected to drought stress imposed either by PEG 6000 solution or water deficit in soil. The survival rates as well as other twelve biological and biochemical indicators such as fresh weight, dry weight, leaf area, relative water content and antioxidant enzyme activity were analyzed under drought stress at seedling stage. Drought tolerance comprehensive evaluation value (D value) and drought resistance coefficient were characterized, which were then integrated into a new index for the identification of the drought tolerant genotype. The results showed that compared with the control, drought severely affected the growth of rapeseed seedlings, and 7 drought tolerant relevant indicators showed significant differences. The shoot fresh weight and leaf area showed significant changes with a respective decrease rate by 69.5% and 57.9%, and POD activity and proline content showed significant increase by 67.2% and 294%, respectively. Base on D value, 8 drought\|tolerant, 39 drought\|sensitive and 32 moderate\|tolerant accessions were identified, which was in good agreement with those assessed by survival rate. Moreover, D value was found to be significantly positively correlated with relative water content (0.221*), shoot fresh weight (0.297**), shoot dry weight (0.253*), root fresh weight (0.321**), root dry weight (0.324**), catalase (CAT) activity (0.276*), POD activity (0.496**), and proline content (0.745**), suggesting that it can be used as a good indicator for drought tolerance screening at seeding stage. |
|
|
|