| Distribution characteristics of soil fungal community in alfalfa field with different standing ages on the rain\|fed Loess Plateau |
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| DOI：10.7606/j.issn.1000-7601.2021.03.20 |
| Key Words: alfalfa growth years fungal community abundance ITS high\|throughput sequencing |
| Author Name | Affiliation | | MA Xin | Collegeof Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou, Gansu 730070, China
Yan’an Institute of Agricultural Sciences, Yan’an, Shaanxi 716000, China | | LUO Zhuzhu | Collegeof Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou, Gansu 730070, China
Gansu Provincial Key Laboratory of Arid Land Crop Science, Lanzhou, Gansu 730070, China | | ZHANG Yaoquan | Collegeof Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | NIU Yining | Gansu Provincial Key Laboratory of Arid Land Crop Science, Lanzhou, Gansu 730070, China | | LI Lingling | Gansu Provincial Key Laboratory of Arid Land Crop Science, Lanzhou, Gansu 730070, China | | CAI Liqun | Collegeof Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou, Gansu 730070, China
Gansu Provincial Key Laboratory of Arid Land Crop Science, Lanzhou, Gansu 730070, China | | CAI Xuemei | Collegeof Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | LIU Jiahe | Collegeof Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou, Gansu 730070, China |
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| Abstract: |
| We selected alfalfa (Medicago sativa) artificial grasslands with three cultivated years (L2003, L2005, and L2012) on the Loess Plateau, with the corn field as control, to explore distribution and succession characteristics of alfalfa soil fungal communities in different growth years, in order to provide a scientific basis for the sustainable use of alfalfa artificial grassland on the Loess Plateau. Soil fungal community was analyzed by sequencing 18s rRNA gene amplicons. The results indicated that 6 phyla, 24 classes, 73 orders, 156 families, and 313 genera of fungi were detected in the loessial soil,including Ascomycota, Zygomycota, and Basidiomycota.The fungal community structures of alfalfa soil and farmland soil were significantly different. As the alfalfa planting period increased, the relative abundance of Ascomycota firstly decreased and then increased, and the relative abundance of Zygomycota and Basidiomycota firstly increased and then decreased. The relative abundance of Ascomycotawas L2003 that was significantly higher than that of L2005 treatment by 12.42%, and the relative abundance of Zygomycota was L2003, significantly lower than that of L2005 treatment by 153.54%. The dominant genera of farmland soil was Mortierella, Metarhizium, and Humicola, and the common dominant genera of alfalfa soil were Phaeomycocentrospora, Mortierella and Beauveria. Compared with alfalfa soil, the relative abundance of Mortierella in farmland soil increased higher significantly by 62.38%~73.48%. With increasing alfalfa growth years, the abundance of probiotic fungi decreased while some pathogenic fungi increased. Principal component analysis showed that Nectria, Ilyonectria, and Fusarium had a high correlation with the first principal component (PC1), while the genera of Lectera, Acremonium, and Gibberella had a high correlation with the second principal component (PC2), and the genera with a high correlation with the first and second principal components were all plant pathogens. |
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