| Screening of biocontrol bacteria against sorghum Fusarium wilt |
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| DOI：10.7606/j.issn.1000-7601.2021.03.30 |
| Key Words: sorghum wilt biocontrol strain antioxidant enzymes soil enzyme |
| Author Name | Affiliation | | MA Wenxu | Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | CHEN Runan | Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | LIU Xinyu | Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | LI Bohao | Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | JIA Wenbao | Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | ZHANG Yanwen | Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | YANG Yan | Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | ZHAO Qianhui | Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | CAO Dongge | Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | YU Gaobo | Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | WEi Jinpeng | Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China |
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| Abstract: |
| Sorghum Fusarium wilt is a serious soil borne disease. In order to inhibit the occurrence of sorghum blight effectively, it is necessary to screen for bio\|control strains with strong inhibitory activity to against the disease. Thirty\|three kinds of bio\|control bacteria were isolated from soil using the method of tablet confrontation in this study, which were infected to sorghum with Fusarium wilt in pot test. Through the sorghum blight disease index and the incidence of a disease, 4 kinds of antagonistic bacteria Y+8, 7, 10+8-20, and Y+6 were selected, with the inhibition rate of the tablet at 83.55%, 85.87%, 85.67% and 89.33%, respectively. The incidence of Fusarium wilt were 37.93%, 30.77%, 30.77% and 42.33%, respectively, which were significantly lower than the control group. The disease indexes were 18.97, 22.12, 30.77, and 16.92, respectively. The molecular identification results showed that the four kinds of antagonistic bacteria with better biocontrol effect were Aquamicrobium, Bacillus, Brevundimonas, and Bacillus. The four biocontrol bacteria induced the production of reactive oxygen species in sorghum leaves, such as H2O2, and improved the activity of antioxidant enzymes significantly, including superoxide dismutase (SOD), peroxidase (POD), ascorbic acid (APX), and also enhanced the activity of defense enzymes, containing phenylalanine ammonialyase (PAL) and polyphenol oxidase (PPO). In addition, the activity of soil urease, phosphatase, polyphenol oxidase, and cellulase were significantly induced by the four biocontrol bacteria. In summary, the biocontrol bacteria, Aquamicrobium, Bacillus, Brevundimonas, and Bacillus could effectively enhance the sorghum defense system, increase the soil enzyme activity, and inhibit the occurrence of sorghum Fusarium wilt, showing a potential application prospect in the biological prevention of sorghum Fusarium wilt. |
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