| Effects of rice planting years on physicochemical properties and bacterial community structure in saline\|alkali soil |
|
View Fulltext View/Add Comment Download reader |
| |
| DOI：10.7606/j.issn.1000-7601.2021.04.25 |
| Key Words: saline\|alkali soil rice planting year bacterial community microbial diversity soil physicochemical properties |
| Author Name | Affiliation | | LI Ming | School of Food and Wine, Ningxia University, Yinchuan, Ningxia 750021, China
Institute of Grape and Wine, Ningxia University, Yinchuan, Ningxia 750021, China | | MA Fei | Institute of Environmental Engineering, Ningxia University, Yinchuan, Ningxia 750021, China | | ZHANG Junhua | Institute of Environmental Engineering, Ningxia University, Yinchuan, Ningxia 750021, China |
|
| Hits: 773 |
| Download times: 157 |
| Abstract: |
| In order to understand the changes of soil microorganism and physicochemical properties during the long\|term rice planting in saline\|alkali soil, this study selected five time series of paddy fields of 0, 1, 6, 12 years and 21 years as treatment groups, and the dryland (corn planting) soil as a control group. The characteristics of bacterial community structure of different rice planting years were analyzed by high\|throughput sequencing on Illumina Miseq platform. At the same time, the relationship between soil physicochemical factors and bacterial community structure was analyzed. The results showed that Proteobacteria, Bacteroides and Actinobacteria were the dominant phylum of soil bacterial community. Among them, Proteobacteria accounted for 36.39%~53.20% of the total amount, and the relative abundance of γ-Proteobacteria in 0-rice planting year was significantly higher than that of other samples (P<0.05). The relative abundance of Proteobacteria was 45.26% in one year of rice planting which was the highest. The relative abundance of Bacteroides was 20.25% and 20.44% in the soil of 12 years and 21 years of rice planting, respectively, which were higher than that of other rice planting years. The relative abundance of Actinobacteria was significantly higher in the soil of 0 year than other samples (P<0.05), which was 17.09%. At the genus level, Brucella was the dominant genus in all sample sites. The results of diversity index showed that the diversity and richness of soil bacterial community in the 12 years of rice planting were significantly lower than those in other samples, which were 17.06%, 85.73% and 82.88% respectively. The results showed that the content of total nitrogen and nitrate was the highest in dryland soil. The microbial biomass carbon and electrical conductivity were the lowest in the 12 years of rice planting, which were lower than those of the control group (101.89%, 23.25%). The content of organic carbon was the highest in 21-year rice soil, which was 29.03%, higher than the control dryland. The pH value was the lowest in 21 years of paddy soil, lower than 1.05% of the control group. Pearson correlation analysis showed that MBC and soil electrical conductivity significantly affected bacterial diversity index. The results of redundancy analysis (RDA) showed that soil electrical conductivity, microbial biomass carbon and nitrate nitrogen were the main factors affecting the bacterial community structure of saline\|alkali rice soil. Our results showed that soil microbial community and physicochemical properties changed significantly with the extension of rice planting years, and the soil habitat showed a significant improvement trend. |
|
|
|