| Polyploid induction of common buckwheat seeds and its C1 generation DNA and morphological identification |
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| DOI：10.7606/j.issn.1000-7601.2021.05.02 |
| Key Words: common buckwheat colchicine polyploid induction C1 generation ploidy identification morphological characteristics |
| Author Name | Affiliation | | LI Deli | College of Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China
State Key Laboratory of Arid Region Crop Adversity Biology College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | DAI Shuangrong | College of Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China
State Key Laboratory of Arid Region Crop Adversity Biology College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | JIA Yanheng | College of Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China
State Key Laboratory of Arid Region Crop Adversity Biology College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | YANG Liming | College of Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China
State Key Laboratory of Arid Region Crop Adversity Biology College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | GAO Jinfeng | College of Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China
State Key Laboratory of Arid Region Crop Adversity Biology College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | WANG Pengke | College of Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China
State Key Laboratory of Arid Region Crop Adversity Biology College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China |
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| Abstract: |
| Using colchicine to induce polyploidy of common buckwheat seeds, two colchicine concentrations of 0.1% (A1) and 0.2% (A2) and five soaking periods of 6h (B1), 9h (B2), 12h (B3), 18h (B4), and 24h (B5) were studied. Polyploid common buckwheat plants were selected by DNA identification of C1 generation plants after seed germination. The characteristics of morphology, stomata, and guard cells were identified. The results showed that the induction rate of A2B3 treatment combination was the highest, which was 80%, followed by A1B5 and A2B5 treatment combination, and the induction rate was 75% and 50%, respectively. The DNA of C1 population was identified by flow cytometry. A total of 65 variant plants were obtained from 193 plantlets, of which 36 were tetraploid and 29 were aneuploid. Morphological identification showed that, compared with the diploid control, the plant height and branches of polyploid common buckwheat decreased and its stem diameter and fibrous root increased. Its root system was strong, leaf surface shrunk rough, and leaf color deepened. The polyploid common buckwheat floral organ became larger and brighter than that of the diploid control common buckwheat. Its seed became larger and the seed coat showed a gray pattern. The stomatal area of lower epidermis of leaves increased, and the length of tetraploid and aneuploid increased by 11.44、20.52 μm. The number of stomata per unit area decreased by 9 and 10, respectively. |
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