| Physiological mechanism of DNA demethylation in improving the cold resistance of Brassica rapa L. |
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| DOI：10.7606/j.issn.1000-7601.2021.05.04 |
| Key Words: Brassica rapa L. cold resistance breeding selection 5-azaC low temperature stress DNA methylation physiological mechanism |
| Author Name | Affiliation | | WANG Wanpeng | College of Agronomy，Gansu Agricultural University, Rapeseed Engineering Research Center of Gansu Province, Key Laboratory of Arid Land Crop Science in Gansu Province, Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou, Gansu 730070, China | | MA Li | College of Agronomy，Gansu Agricultural University, Rapeseed Engineering Research Center of Gansu Province, Key Laboratory of Arid Land Crop Science in Gansu Province, Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou, Gansu 730070, China | | SUN Bolin | College of Agronomy，Gansu Agricultural University, Rapeseed Engineering Research Center of Gansu Province, Key Laboratory of Arid Land Crop Science in Gansu Province, Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou, Gansu 730070, China | | GUO Xiujuan | Shanxi Academy of Agricultural Sciences Crop Research Institute of High and Cold Regions, Datong, Shanxi 037000, China | | WANG Shifa | Jilin Academy of Agricultural Sciences Economic Plant Development and Research Center, Changchun, Jilin 130124, China | | NIU Zaoxia | College of Agronomy，Gansu Agricultural University, Rapeseed Engineering Research Center of Gansu Province, Key Laboratory of Arid Land Crop Science in Gansu Province, Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou, Gansu 730070, China | | QI Weiliang | College of Agronomy，Gansu Agricultural University, Rapeseed Engineering Research Center of Gansu Province, Key Laboratory of Arid Land Crop Science in Gansu Province, Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou, Gansu 730070, China | | PU Yuanyuan | College of Agronomy，Gansu Agricultural University, Rapeseed Engineering Research Center of Gansu Province, Key Laboratory of Arid Land Crop Science in Gansu Province, Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou, Gansu 730070, China | | LU Xiaoming | College of Agronomy，Gansu Agricultural University, Rapeseed Engineering Research Center of Gansu Province, Key Laboratory of Arid Land Crop Science in Gansu Province, Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou, Gansu 730070, China | | HU Fangdi | College of Agronomy，Gansu Agricultural University, Rapeseed Engineering Research Center of Gansu Province, Key Laboratory of Arid Land Crop Science in Gansu Province, Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou, Gansu 730070, China | | XU Jinmiao | College of Agronomy，Gansu Agricultural University, Rapeseed Engineering Research Center of Gansu Province, Key Laboratory of Arid Land Crop Science in Gansu Province, Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou, Gansu 730070, China | | MA Xuecai | College of Agronomy，Gansu Agricultural University, Rapeseed Engineering Research Center of Gansu Province, Key Laboratory of Arid Land Crop Science in Gansu Province, Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou, Gansu 730070, China | | WU Junyan | College of Agronomy，Gansu Agricultural University, Rapeseed Engineering Research Center of Gansu Province, Key Laboratory of Arid Land Crop Science in Gansu Province, Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou, Gansu 730070, China | | LI Xuecai | College of Agronomy，Gansu Agricultural University, Rapeseed Engineering Research Center of Gansu Province, Key Laboratory of Arid Land Crop Science in Gansu Province, Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou, Gansu 730070, China | | FANG Yan | College of Agronomy，Gansu Agricultural University, Rapeseed Engineering Research Center of Gansu Province, Key Laboratory of Arid Land Crop Science in Gansu Province, Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou, Gansu 730070, China | | SUN Wancang | College of Agronomy，Gansu Agricultural University, Rapeseed Engineering Research Center of Gansu Province, Key Laboratory of Arid Land Crop Science in Gansu Province, Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou, Gansu 730070, China | | LIU Lijun | College of Agronomy，Gansu Agricultural University, Rapeseed Engineering Research Center of Gansu Province, Key Laboratory of Arid Land Crop Science in Gansu Province, Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou, Gansu 730070, China |
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| Abstract: |
| Ten Brassica rapa materials from different breeding environments were used to analyze the relationship between cold resistance and environment by measuring semi\|lethal temperature. Treated with different concentrations of DNA methylation inhibitor 5-azaC, the effects of DNA demethylation on DNA methylation level and seedling physiological characteristics of Brassica rapa L. under low temperature stress were examined. The results showed that the semi\|lethal temperatures of materials selected in different breeding environments were significantly different, and the semi\|lethal temperatures of 2018-FJT, DT-7, DT-9, and MXW-1 were -16.04℃, -15.98℃, -15.63℃, and -15.04℃, respectively. The semi\|lethal temperatures of CT-2360, CT-2380, CT-2400, CT-2420, CT-2440, and CT-2460 were -11.32℃, -11.6℃, -11.42℃, -11.44℃, -12.97℃ and -13.28℃, respectively. According to the semi\|lethal temperature, the cold resistance of 10 Brassica rapa L. was in the following order: 2018-FJT>DT-7>DT-9>MXW-1>CT-2460>CT-2400>CT-2380>CT-2420>CT-2440>CT-2360. The formation of cold resistance was positively correlated with the latitude and annual average temperature of the breeding environment. Four materials CT-2360, MXW-1, 2018-FJT, and DT-7 with cold resistance and different producing areas were selected for physiological determination. The results showed that 1 000 μmol·L-1 5-azaC treatment could significantly inhibited the root growth of seedlings and the root length of the four materials decreased by 93.14% and 95.06% compared with the control. Under low temperature, 5-azaC treatment had the most significant effects on the REC and MDA content of CT-2360 seedlings, as well as the Pro and soluble protein content of strong cold resistant material DT-7. During the low temperature treatment, the SOD, POD, and CAT activities of the four materials all increased, and the low temperature treatment for 5 days was the most significant (P<0.05). Of which the SOD activity of 2018-FJT increased the most, which was 45.85%. The POD of DT-7 increased the most with 460%. The CAT of CT-2360 increased most significantly with 321.02%. HPLC analysis found that the methylation level of CT-2360 in materials with weak cold resistance at room temperature was 77.48%, which was higher than that of the other three materials with strong cold resistance. Significant demethylation occurred after 5-azaC treatment. This study supports that the cold resistance is regulated by DNA demethylation. |
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