| ]Effects of potassium\|solubilizing bacteria and salt stress on root property and physiological metabolism of Lycium barbarum seedlings |
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| DOI：10.7606/j.issn.1000-7601.2021.05.06 |
| Key Words: potassium\|solubilizing bacteria salt stress Lycium barbarum seedlings root characteristics physiological metabolism |
| Author Name | Affiliation | | ZHU Juanjuan | College of Biological Science and Engineering,North Minzu University, Yinchuan, Ningxia 750021, China
Ningxia Grape & Wine Innovation Center, Yinchuan, Ningxia 750021
China | | MA Haijun | College of Biological Science and Engineering,North Minzu University, Yinchuan, Ningxia 750021, China
Ningxia Grape & Wine Innovation Center, Yinchuan, Ningxia 750021
China | | QIN Hongyun | College of Biological Science and Engineering,North Minzu University, Yinchuan, Ningxia 750021, China | | XU Xiaoyun | College of Biological Science and Engineering,North Minzu University, Yinchuan, Ningxia 750021, China | | ZHANG Xiu | College of Biological Science and Engineering,North Minzu University, Yinchuan, Ningxia 750021, China
Key Laboratory of Microbial Resources Development and Utilization in Ningxia Special Habitat, Yinchuan, Ningxia 750021, China |
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| Abstract: |
| Cultivar Ningqi 1 was used to investigate the regulating effects of salt stress (100 mmol·L-1 NaCl) and potassium\|solubilizing bacteria (B1, B2, B3) on total root length, root projected area, root surface area, root volume, root average diameter, root tip number, root activity, superoxide anion (O—·2) content, hydrogen peroxide (H2O2) content, soluble sugar content and superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), glutathione peroxidase (GSH-PX), as well as sucrose phosphate synthase (SPS), sucrose synthase (SS), and invertase (INV) activity of Lycium barbarum seedlings. Double factor fracture zone test design was employed to evaluate comprehensively the root growth status of Lycium barbarum seedlings in different treatments with salt stress as the main factor and potassium\|solubilizing bacteria as the blessing factor. Results showed that potassium\|solubilizing bacteria and salt stress had no significant effect on the content of superoxide anion (O—·2), the activity of SOD, CAT, and POD in the roots of Lycium barbarum seedlings, but had significant effect on other indicators. Among different treatment combinations, the root length of Lycium barbarum seedlings was the longest in B1 treatment, which was longer by 101%, 89%, 65%, 112%, 105%, 122%, and 181% than that of ck, B2, B3, NaCl-CK, NaCl-B1, NaCl-B2, and NaCl-B3 treatments, respectively. The projected area, surface area, volume, and invertase activity of roots were the highest in B2 treatment. The root tip number, root activity, GSH-PX activity and SS activity were the highest in NaCl-B1 treatment. The soluble sugar content and SPS activity were the highest in NaCl-B2 treatment. The H2O2 content in the roots of Lycium barbarum seedlings was the lowest in NaCl-B3 treatment, which was lower by 24.3%, 34.3%, 3.9%, 41.1%, 17.3%, 36.3%, and 14.3% than that of ck, NaCl-CK, B1, NaCl-B1, B2, NaCl-B2, and B3 treatments, respectively. The comprehensive evaluation score by principal component analysis showed that the top three treatments were NaCl-B1, B2, and NaCl-B2. Generally, potassium\|solubilizing bacteria promoted the growth and development of the root system of Lycium barbarum seedlings. Whether under salt stress or not, inoculation of single strains B1 or B2 was better than that of mixed strains （B3）. Inoculation of potassium\|releasing bacteria B1 was more conducive to the growth and development of Lycium barbarum roots under salt stress. Inoculation of B2 potassium\|solubilizing bacteria was more conducive to the growth and development of the roots of Lycium barbarum seedlings under non\|salt stress. |
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