| Selecting drought resistance indexes of Brassica napus L. under PEG 6000 simulated drought stress at germination and seedling stage |
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| DOI：10.7606/j.issn.1000-7601.2021.05.08 |
| Key Words: Brassica napus L. PEG 6000 drought resistance index germination stage seedling stage |
| Author Name | Affiliation | | LIU Xiang | College of Agronomy, Northwest A & F University, Yangling, Shaanxi 712100, China | | ZUO Kaifeng | College of Agronomy, Northwest A & F University, Yangling, Shaanxi 712100, China | | XU Wei | Mian County Original Seed Farm, Mian County, Shaanxi 724200, China | | GUO Na | College of Agronomy, Northwest A & F University, Yangling, Shaanxi 712100, China | | QIN Mengfan | College of Agronomy, Northwest A & F University, Yangling, Shaanxi 712100, China | | XU Yu | College of Agronomy, Northwest A & F University, Yangling, Shaanxi 712100, China | | MA Ning | College of Agronomy, Northwest A & F University, Yangling, Shaanxi 712100, China | | LI Qingqing | College of Agronomy, Northwest A & F University, Yangling, Shaanxi 712100, China | | ZHANG Miao | College of Agronomy, Northwest A & F University, Yangling, Shaanxi 712100, China | | LI Haodong | College of Agronomy, Northwest A & F University, Yangling, Shaanxi 712100, China | | WANG Rongrong | College of Agronomy, Northwest A & F University, Yangling, Shaanxi 712100, China | | HUANG Zhen | College of Agronomy, Northwest A & F University, Yangling, Shaanxi 712100, China | | XU Aixia | College of Agronomy, Northwest A & F University, Yangling, Shaanxi 712100, China |
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| Abstract: |
| Polyethylene glycol 6000 (PEG 6000) was used to simulate drought stress, analyze the drought resistance related indexes of eight Brassica napus L. germplasms at germination and seedling stage, identify drought resistance of test materails and select for drought resistance evaluation indexes. The results showed that the drought resistance of Brassica napus L. was different at germination stage and seedling stage. The optimum PEG 6000 concentration for drought resistance identification at germination stage was 15%, and the seedling rate could be used as an indicator of drought resistance at germination stage. Component analysis, membership function and other methods were used to analyze the changes of relevant indicators at seedling stage. The drought resistance of Brassica napus L. at seedling stage was evaluated by the comprehensive measure of drought resistance D value. The results showed that the relative water content of leaves (r=0.907**) and soluble protein (r=0.921**) were significantly positively correlated with D value, and malondialdehyde content was significantly negatively correlated with the D value (r=-0.837**). These indicators could be used as drought resistance evaluation indicators in seedling stage of Brassica napus L. |
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