| Effects of 2，4-epibrassinolide on antioxidant system of grape seedlings treated with carbendazim |
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| DOI：10.7606/j.issn.1000-7601.2021.05.09 |
| Key Words: wine grape 2,4-epibrassinolide carbendazim antioxidant system |
| Author Name | Affiliation | | SUN Jiahua | College of Enology, Northwest A & F University, Yangling, Shaanxi 712100, China | | LI Yumeng | College of Enology, Northwest A & F University, Yangling, Shaanxi 712100, China | | LING Han | College of Enology, Northwest A & F University, Yangling, Shaanxi 712100, China | | XI Zhumei | College of Enology, Northwest A & F University, Yangling, Shaanxi 712100, China |
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| Abstract: |
| This study was conducted to determine the influence of exogenous EBR (2，4-Epibrassinolide) on antioxidant system of grape seedlings treated with carbendazim (CBD). With wine grape variety Cabernet Sauvignon (Vitis vinifera L.) biennial potted seedlings as the experimental material, effects of the exogenous EBR on reactive oxygen species (H2O2, O—·2), malondialdehyde content (MDA), antioxidant substances content (ASA, GSH) and antioxidant enzyme activities (CAT, POD, GR) of grape leaves were investigated. There were three treatments: CK(water), 800 times diluted CBD, 0.3 mg·L-1 EBD+800 times diluted CBD. As compared with control, CBD treatment increased the content of H2O2, MDA, and O—·2 in grape leaves, and on average it increased by 50.09%, 35.58%, and 60.06%, respectively. There was no significant difference in the content of antioxidant substances GSH and AsA in grape leaves when compared to control group. In comparison with control, CBD treatment caused significant enhance of CAT, POD, and GR activities in grape leaves, and on average it increased by 24.17%, 63.51%, 8.44%, respectively. When compared to CBD treatment alone, EBR+CBD treatment significantly decreased the content of H2O2, MDA, and O—·2, averagely decreased by 18.49%, 14.44%, and 17.55%, respectively. EBR+CBD treatment significantly increased the content of ASA and GSH in grape leaves compared to CBD treatment alone, and the average increases were 5.46% and 24.92%, respectively. In comparison with CBD treatment alone, EBR+CBD treatment caused significant enhance of CAT and GR activities in grape leaves, averagely increased by 17.15%, 5.33%, respectively, while did not cause an obvious effect on POD activity. Therefore, 0.3 mg·L-1 exogenous EBR decreased the level of endogenous reactive oxygen species and free radicals by probably increasing the content of reduced antioxidants and the activities of CAT and GR in grape plants to alleviate the damage of pesticides to grape plants. This method can be used as a pretreatment in grape production. |
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