| Diversity analysis of soil bacterial community in oil flax\|wheat rotation system |
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| DOI：10.7606/j.issn.1000-7601.2021.05.11 |
| Key Words: oil flax\|wheat rotation soil bacteria community high\|throughput sequencing diversity analysis Tax4Fun function prediction |
| Author Name | Affiliation | | WANG Liguang | Biotechnology Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu 730070, China | | YE Chunlei | Biotechnology Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu 730070, China | | CHEN Jun | Biotechnology Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu 730070, China | | Li Jinjing | Biotechnology Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu 730070, China | | LI Jingwen | Biotechnology Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu 730070, China | | LUO Junjie | Biotechnology Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu 730070, China |
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| Abstract: |
| In order to reveal the effect of flax\|wheat rotation on the structure and diversity of soil bacterial community, the high\|throughput sequencing technique was used to determine the bacterial community indifferent soil collected. The results showed that the number of bacteria OTU (1778) was the highest, and the abundance index（Chao index 1700.02 and Ace index 1689.03）of bacteria community was the lowest in the TC2 soil samples. The diversity of bacterial community (Shannon index TC1 (6.51）＞TR（6.42）＞TC2(6.21), Simpson index TC2 (0.0088）＞TR（0.0049）＞TC1(0.0030)) decreased with replacement years. A total of 33 phylum of bacteria were isolated from the different soil samples. The 11-dominant phylum（＞0.01）were the same in different samples, including proteobacteria, acidobacteria, actinobacteria and chloflexi, etc. The abundance of the dominant phylum was different in each sample. With the replacement of planting, the abundance of proteobacteria (TC1 31.65%, TR 24.52%, TC2 28.53%) and gemmatimonadetes (TC1 8.04%, TR 5.41%, TC2 6.01%) decreased first and then increased. The abundance of acidobacteria (TC1 19.36%, TR 26.13%, TC2 11.18%) and chloroflexi (TC1 9.69%, TR 12.68%, TC2 10.53%) increased first and then decreased. The abundance of actinobacteria (TC1 12.14%, TR 13.79%, TC2 22.82%) increased gradually. Beta diversity analysis of bacterial community showed that wheat rotation had little effect on the structure of soil bacterial community like TC1, but the follow\|up cultivation of oil flax had more effect. Cell metabolism, genetic information processing and environmental information processing were detected by Tax4Fun method in different soil collected. The abundance of most functional genes in TR was similar to that of TC1. All the results in this experiment indicated that wheat rotation helped maintain diversity and structure of soil bacteria community and change soil bacteria community slightly, so as to benefit the cultivation of subsequently oil flax. These results provided theoretical basis for the mechanism analysis of relieving continuous cropping obstacle of oil flax by rotation. |
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