| Function analysis of MdGH3-2/12 under salt stress in apple |
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| DOI：10.7606/j.issn.1000-7601.2021.06.05 |
| Key Words: MdGH3-2/12 apple salt stress function analysis |
| Author Name | Affiliation | | MA Mengnan | College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China | | LIU Yuan | College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China | | MA Fengwang | College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China | | ZOU Yangjun | College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China |
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| Abstract: |
| Conducted during May 2020 to February 2021, this experiment used apple wild\|type WT plants and MdGH3-2/12 RNA interference transgenic plants (RNAi) as materials to explore the function of auxin response gene MdGH3-2/12 to salt stress of apple. Changes of phenotype, growth, photosynthetic parameters, chlorophyll content, antioxidant enzymes and reactive oxygen species (ROS) content of apple plants under salt stress were evaluated.The research results are as follows:(1) Compared with WT, RNAi plants （lines of GR-1, GR-9, GR-10） showed more brown spots and necrosis on leaf surface, and root activity was significantly reduced, which were 93.8%, 77.5% and 90.0% of the WT plants, respectively. The growth and photosynthesis of the RNAi plants were significantly affected,and Pn, Ci and Gs were significantly decreased, with Pn values dropping to 62.9%, 77.41% and 83.6% of the WT plants, respectively. The maximum photochemical efficiency (Fv/Fm) decreased to 97.0%, 96.4% and 97.5% of the WT plants, respectively. Chlorophyll content decreased significantly to 90.7%, 86.2% and 81.9% of the WT plants, MDA was 1.15, 1.25 and 1.16 times of the WT plants, and REL was 1.23, 1.39 and 1.48 times of the WT plants.The stomatal opening of leaves was decreased by 35.0%, 39.0%, 55.0% and 46.2%, respectively, under salt stress. (2) Compared with WT, Pro content in RNAi lines increased significantly, which was 7.1, 10.3 and 6.3 times of the WT plants. ROS accumulation in leaves was significantly higher than that in WT. H2O2 content in all lines was 1.6, 1.8 and 1.8 times of that in the WT plants, while antioxidant enzyme activity was significantly decreased.For example, SOD activity was 2.4, 1.2, 1.7 and 1.9 times of the control group, respectively, indicating that RNAi strains didn’t effectively remove ROS under salt stress and could cause more damage to plants. (3) Compared with WT, the Na+/K+ ratio of plants under salt stress were 0.497, 0.558, 0.525 and 0.577, which was significantly higher than that of WT (0.497). However, the expression level of related ion transporter genes was significantly lower than that of the WT plants. As a result, Na+ was unable to be discharged in time, and the plants were poisoned by sodium ions, which led to the decrease of salt tolerance. These results indicate that MdGH3-2/12 gene plays an important role in the salt stress response of apple plants. After interference with MdGH3-2/12 gene, the apple plants suffered more damage under salt stress, and the resistance of apple plants to salt stress decreased. |
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