| Effects of growth years on soil bacterial community structure in rhizosphore soil of Fritillaria przewalskii |
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| DOI：10.7606/j.issn.1000-7601.2021.06.17 |
| Key Words: Fritillaria przewalskii high\|throughput sequencing bacterial community diversity |
| Author Name | Affiliation | | WU Rui | College of Agronomy, Gansu Agricultural University, Gansu Provincial Key Laboratory of Good Agricultural Production for Traditional Chinese Medicines, Gansu Provincial Engineering Research Centre for Medical Plant Cultivation and Breeding, Lanzhou, Gansu 730070, China
Pharmacy Department, Gansu University of Chinese Medicine, Dingxi, Gansu 743000, China | | CHEN Yuan | College of Agronomy, Gansu Agricultural University, Gansu Provincial Key Laboratory of Good Agricultural Production for Traditional Chinese Medicines, Gansu Provincial Engineering Research Centre for Medical Plant Cultivation and Breeding, Lanzhou, Gansu 730070, China | | GUO Fengxia | College of Life Science and Technology, Gansu Agricultural University, Gansu Key Laboratory of Crop Genetic & Germplasm Enhancement, Gansu Provincial Key Laboratory of Arid Land Crop Science, Lanzhou, Gansu 730070, China | | ZHOU Yang | College of Agronomy, Gansu Agricultural University, Gansu Provincial Key Laboratory of Good Agricultural Production for Traditional Chinese Medicines, Gansu Provincial Engineering Research Centre for Medical Plant Cultivation and Breeding, Lanzhou, Gansu 730070, China | | JIAO Xusheng | College of Agronomy, Gansu Agricultural University, Gansu Provincial Key Laboratory of Good Agricultural Production for Traditional Chinese Medicines, Gansu Provincial Engineering Research Centre for Medical Plant Cultivation and Breeding, Lanzhou, Gansu 730070, China |
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| Abstract: |
| In this study, the V3-V4 aera of 16S rRNA gene of bacteria from the Fritillaria przewalskii rhizosphere soil with cultivated land abandonment (CK), 1 year (BM-1Y), 3 years (BM-3Y) and 5 years (BM-5Y) were sequenced by high\|throughput sequencing, and the effect of different growth years of Fritilaria przewalskii on the bacterial community structure of rhizosphere soil was discussed. The results showed that the growth years of Fritillaria przewalskii had an impact on both physical and chemical properties and bacterial consortium of soil. BM-5Y treatment significantly reduced organic matter (OM), hydrolyzed nitrogen (HN) and available phosphorus (AP) content (P<0.05) by 22.76%, 9.28%, and 51.25% respectively when compared with CK. BM-5Y significantly reduced the bacterial diversity of the rhizosphere soil (P<0.05). Of which, the OUT number, PD value and Chao I index were reduced by 7.23%, 6.79%, and 6.47% respectively compared with CK. Growth years changed the composition of bacterial communities at the phylum and genus level. However, the dominant bacteria phyla (relative abundance>5%) were consistent in all treatments, and they were Actinobacteria, Proteobacteria, Acidobacteria and Chloroflexi. The sum of relative abundances in CK, BM-1Y, BM-3Y and BM-5Y were 82.28%, 84.16%, 83.12% and 82.30%, respectively. Soil pH, organic matter, hydrolyzed nitrogen, available phosphorus, and available potassium content were the important driving factors the bacterial community in the rhizosphere, and their degree was: pH＞AK＞HN＞AP＞OM. The physicochemical properties of soil had different correlations with the dominant bacteria phyla. pH was significantly negatively correlated with Actinobacteria and Gemmatimonadetes (P<0.05). Organic matter, hydrolyzed nitrogen and available phosphorus were significantly negatively correlated withFirmicutes (P<0.05). In summary, the nutrient content and diversity of the bacterial communities of the soil were significantly reduced with the extension of growth years (BM-5Y) in Fritillaria przewalskii. |
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