| Isolation and identification as well as growth enhancement and saline\|alkali tolerance properties of Cerasus humilis plant growth\|promoting endophytes |
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| DOI：10.7606/j.issn.1000-7601.2022.01.15 |
| Key Words: Cerasus humilis plant growth\|promoting endophytes nitrogen\|fix phosphorus\|dissolving phosphorus\|solubilizing, plant growth hormone (IAA) saline\|alkali tolerance |
| Author Name | Affiliation | | BAI Jie | Key Laboratory of Grassland Ecosystem, Pratacultural College, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | YAO Tuo | Key Laboratory of Grassland Ecosystem, Pratacultural College, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | WANG Zhanjun | Desertification Control Institute of Ningxia Academy of Agriculture and Forestry Science, Yinchuan, Ningxia 750000, China | | LEI Yang | Key Laboratory of Grassland Ecosystem, Pratacultural College, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | YANG Xiaolei | Key Laboratory of Grassland Ecosystem, Pratacultural College, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | ZHANG Wei | Key Laboratory of Grassland Ecosystem, Pratacultural College, Gansu Agricultural University, Lanzhou, Gansu 730070, China |
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| Abstract: |
| To obtain Cerasus humilis root plant growth\|promoting endophytes, PGPE, we took the root system of Cerasus humilis as the research object and used tissue homogenization method to isolate and purify cultivable endophytic bacteria to determine its nitrogen fixation, phosphorus\|dissolving, phosphorus\|solubilizing, IAA production and saline\|alkali tolerance properties. We also tried to use phylogenetic analysis of 16S rRNA sequence for preliminary identification. The results showed that the 7 isolated strains all showed a certain growth potential. The nitrogen fixation activity of strains was within the range between 50.64~127.40 nmol·mL-1, and the Y3 strain had the highest nitrogen fixation activity. The dissolution of organic phosphorus strains was within the range between 1.28~358.90 μg·mL-1, and the N23 strain had the highest capacity of dissolving organic phosphorus. The solubilization of inorganic phosphorus strains was within the range between 6.29~143.61 μg·mL-1, and the Y2 strain had the highest capacity of solubilizing inorganic phosphorus. The content of IAA synthesized by the strain was between 14.24 μg·mL-1 and 70.48 μg·mL-1, and the content of IAA synthesized by the N22 strain was the highest. In addition, the Y3 and Y4 strains showed a wide range of saline\|alkali tolerance characteristics and grew normally under the condition of 30% NaCl concentration and pH of 13. They were extremely halophilic and alkalophilic micro\|organisms. As a result of phylogenetic analysis of 16S rRNA sequence, the Y3 and Y4 strains were identified as Bacillus sp. N23 and N22 strains were Pseudomonas simiae. Strain N1 was Brevibacterium frigoritolerans. The Y1 and Y2 strains were Achromobacter marplatensis. The PGPE isolated in this study contained a variety of high\|quality growth\|promoting properties, which could provide excellent strains for the subsequent development of microbial inoculants (bacterial fertilizers) and play an active role in the development of green agriculture. |
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