| Physiological response of alfalfa cold resistance to types and rates of fall potassium fertilizer application |
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| DOI：10.7606/j.issn.1000-7601.2022.01.28 |
| Key Words: potassium fertilizer fertilizer amount type alfalfa cold resistance physiological response |
| Author Name | Affiliation | | SUN Mingxue | College of Agriculture, Inner Mongolia Minzu University/Inner Mongolia Autonomous Region Feedstuff Engineering Technology Research Center, Tongliao,Inner Mongolia 028041, China | | ZHANG Yuxia | College of Agriculture, Inner Mongolia Minzu University/Inner Mongolia Autonomous Region Feedstuff Engineering Technology Research Center, Tongliao,Inner Mongolia 028041, China | | CONG Baiming | Institute of Animal Husbandry and Veterinary Science, Tongliao, Inner Mongolia 028000, China | | XIA Quanchao | College of Agriculture, Inner Mongolia Minzu University/Inner Mongolia Autonomous Region Feedstuff Engineering Technology Research Center, Tongliao,Inner Mongolia 028041, China | | TIAN Yonglei | Academy of Agriculture and Animal Husbandry, Hohhot, Inner Mongolia 010010, China | | ZHANG Qingxin | College of Agriculture, Inner Mongolia Minzu University/Inner Mongolia Autonomous Region Feedstuff Engineering Technology Research Center, Tongliao,Inner Mongolia 028041, China | | DU Xiaoyan | College of Agriculture, Inner Mongolia Minzu University/Inner Mongolia Autonomous Region Feedstuff Engineering Technology Research Center, Tongliao,Inner Mongolia 028041, China |
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| Abstract: |
| By analyzing the physiological characteristics of overwintering organs of alfalfa cultured with different potassium fertilizers under low temperature treatment,the response of the cold resistance of alfalfa to the types and amounts of potassium fertilizer applied during fall was discussed.In this experiment,the ‘Gibraltar’ alfalfa variety was used as the test material.Two kinds of potassium fertilizers, K2SO4 and KCl, were applied in fall, and the application rates were 0,50,100,150,200 kg·hm-2K2O (indicated by K0, K1, K2, K3, K4). The overwintering materials were excavated in the early stage of overwintering of alfalfa,and subjected to simulated low temperature treatment (4℃,-20℃) to determine the relative conductivity of the alfalfa root neck,free water,bound water,proline,and free amino acid content.The results showed that for KCl and K2SO4,the free water content of alfalfa root neck at -20℃ was reduced by 11%～26% and 9%～51%, respectively,compared with the cold storage treatment at 4℃, and free water/bound water were reduced by 15.09%～53.56% and 22.95%～71.98% respectively compared with 4℃ treatment. For KCl and K2SO4,the relative conductivity of alfalfa root neck under -20℃ treatment increased by 129.70%～170.10% and 53.39%～143.52%,respectively,compared with 4℃ treatment. The content of bound water was higher than 4℃ treatment and increased by 4.89%～88.21% and 16.30%～164.49%, respectively. Free amino acid content increased by 7.01%～50.32% and 6.40%～82.69% compared with 4℃ treatment respectively. The proline content increased by 1.71%～18.10% and 3.40%～29.67%,respectively,compared with the treatment at 4℃. The content of bound water, free amino acid and proline in the root neck of alfalfa treated with K2SO4 were higher than that of KCl treatment under the low temperature freezing treatment at -20℃. The bound water content and free amino acid content of the K3 treatment were 150 kg·hm-2 K2O. Compared with the KCl treatment, the content and the proline content increased by 12.50%,22.32% and 1.53%, respectively. Compared with the KCl treatment, the free water content and free water/bound water decreased by 14.91% and 24.71%, respectively. Therefore, the application of 150 kg·hm-2 K2O of K2SO4 in fall was most conducive to the safe overwintering of alfalfa as it increased the content of free amino acids and proline osmotic adjustment substances,reduced the free water content and the free water/bound water ratio,and improved the cold resistance of alfalfa. |
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