Drought resistance evaluation of 23 ryegrass germplasms at germination stage
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DOI:10.7606/j.issn.1000-7601.2022.02.01
Key Words: ryegrass  Lolium multiflorum  germination stage  drought resistance evaluation  PEG-6000
Author NameAffiliation
CHENG Shaobo Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China 
YANG Xunzhe Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China 
ZOU Li Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China 
WU Dandan Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan 611130, China 
LU Jiale College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan 611130, China 
ZHOU Yonghong Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan 611130, China 
ZHANG Haiqin College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan 611130, China 
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Abstract:
      To explore the drought resistance ability of different ploidy ryegrass (Lolium multiflorum) and screen excellent germplasm resources, 23 ryegrass germplasms were used to evaluate drought resistance during seed germination under PEG-6000 simulated drought conditions in this study. Six indexes, such as relative root length, relative shoot length, and relative germination rate were measured. The drought tolerance of ryegrass was comprehensively evaluated by membership function analysis, principal component analysis and cluster analysis. The results showed that the root length and shoot length of tetraploid ryegrass were 1.79 times and 1.26 times higher than that of diploid ryegrass under drought treatment, respectively. Principal component analysis showed that vigor index and root length were the main components of germination factor and root growth factor, respectively, and the loading capacity was 0.924 and 0.743, respectively. The D value of 23 materials ranged from 0.117 to 0.946, among which the D value of PI 238886 was the lowest while that of PI 343155 was the highest. Five tetraploid drought resistant materials (PI 343155, Tetragold, 929-3, Chuannong No.1, PI 619469) and five diploid drought resistant materials (PI 266111, PI 283610, PI 619471, PI 634251, W6 9271) were selected by cluster analysis. Nine medium and 4 drought sensitive materials were also identified in this study.