| Research on drought\|tolerant regulatory mechanism of maize roots using transcriptome and iTRAQ techniques |
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| DOI：10.7606/j.issn.1000-7601.2022.02.08 |
| Key Words: maize root system transcriptome proteome joint analysis drought\|tolerant mechanism |
| Author Name | Affiliation | | BAI Mingxing | College of Agronomy, Gansu Agricultural University, Gansu Provincial Key Lab of Arid Land Crop Science， Gansu Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou, Gansu 730070, China | | ZHUANG zelong | College of Agronomy, Gansu Agricultural University, Gansu Provincial Key Lab of Arid Land Crop Science， Gansu Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou, Gansu 730070, China | | JI Xiangzhuo | College of Agronomy, Gansu Agricultural University, Gansu Provincial Key Lab of Arid Land Crop Science， Gansu Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou, Gansu 730070, China | | PENG Yunling | College of Agronomy, Gansu Agricultural University, Gansu Provincial Key Lab of Arid Land Crop Science， Gansu Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou, Gansu 730070, China |
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| Abstract: |
| RNA-Seq and iTRAQ techniques were used to analyze the association of differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) in seedling roots of two maize inbred lines, Chang 7-2 and TS141, with different drought tolerance. The results of quantitative correlation analysis showed that the correlation coefficients of genes with the same trend of mRNA and protein expression in Chang 7-2 D1 vs CK1, Chang 7-2 D2 vs CK2, TS141 D1 vs CK1 and TS141 D2 vs CK2 were 0.7993, 0.5673, 0.6406, and 0.6588, respectively. The correlation coefficients of mRNA and genes with opposite protein expression trend were -0.7222, -0.5752, -0.7996, and-0.7339, respectively. Further, by integrating the association of each self\|crossing D1 vs CK1 and D2 vs CK2, it was found that there were 19 genes with the same expression trend of mRNA and protein in Chang 7-2, 9 gene with opposite expression trend of mRNA and protein, 11 genes with the same expression trend of mRNA and protein in TS141, and 0 gene with opposite expression trend of mRNA and protein, indicating that there were differences in transcriptional and post\|transcriptional regulation in plant roots. KEGG analysis showed that the DEGs/DEPs associated with the four comparison groups were mainly related to phenylpropanoid biosynthesis, biosynthesis of secondary metabolites and ribosome, and these pathways might be the main pathways in response to drought stress in the roots of different inbred lines. 9 genes with the same expression trend of DEPs/DEGs were selected from Chang 7-2 and TS141 for qRT-PCR analysis, and the results showed that the expression trend of 9 DEPs/DEGs was basically consistent with their RNA-Seq and iTRAQ results. |
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