| Effects of long\|term combined application of nitrogen and phosphorus on bacterial diversity and soil enzyme activities in cinnamon soil |
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| DOI：10.7606/j.issn.1000-7601.2022.02.20 |
| Key Words: bacterial diversity enzyme activity combined application of nitrogen and phosphorus cinnamon soil maize |
| Author Name | Affiliation | | LIU Zhiping | Institute of Resources and Environment, Shanxi Agricultural University, State Key Laboratory of Sustainable Dryland Agriculture（in preparation）, Taiyuan, Shanxi 030031, China | | ZHOU Huaiping | Institute of Resources and Environment, Shanxi Agricultural University, State Key Laboratory of Sustainable Dryland Agriculture（in preparation）, Taiyuan, Shanxi 030031, China | | XIE Wenyan | Institute of Resources and Environment, Shanxi Agricultural University, State Key Laboratory of Sustainable Dryland Agriculture（in preparation）, Taiyuan, Shanxi 030031, China | | YANG Zhenxing | Institute of Resources and Environment, Shanxi Agricultural University, State Key Laboratory of Sustainable Dryland Agriculture（in preparation）, Taiyuan, Shanxi 030031, China | | MA Xiaonan | Institute of Resources and Environment, Shanxi Agricultural University, State Key Laboratory of Sustainable Dryland Agriculture（in preparation）, Taiyuan, Shanxi 030031, China | | HU Xuechun | Institute of Resources and Environment, Shanxi Agricultural University, State Key Laboratory of Sustainable Dryland Agriculture（in preparation）, Taiyuan, Shanxi 030031, China |
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| Abstract: |
| To explore the effects of different combinations of nitrogen and phosphorus fertilizer application on soil bacterial community structure and enzyme activities, five fertilization treatments were set up based on the long\|term fertilization experiment in cinnamon soil of Shouyang, Shanxi Province. The treatments included CK (no fertilizer), N1P1 (N: 60 kg·hm-2; P: 37.5 kg·hm-2), N2P2 (N: 120 kg·hm-2; P: 75 kg·hm-2), N3P3 (N: 180 kg·hm-2; P: 112.5 kg·hm-2) and N4P4 (N: 240 kg·hm-2; P: 150.0 kg·hm-2). Soil samples from the tillage layer (0~20 cm) after maize harvest were collected, and the V3-V4 region of soil bacteria 16S rRNA was sequenced by Illumina HiSeq high\|throughput sequencing technology. The activities of soil sucrase, urease and alkaline phosphatase were measured by colorimetric method, and the correlation between bacterial community structure and soil enzyme activities was analyzed. The results showed that the combined application of nitrogen and phosphorus increased soil nutrients and maize yield. Compared with CK, the yield of maize under N1P1, N2P2, N3P3 and N4P4 treatments increased by 3.3%~60.9%, 120.1%~862.6%, 7.9%~18.4% and 63.9%~86.1%, respectively. With the increase of nitrogen and phosphorus application rates, bacterial α-diversity reached the highest in N1P1 treatment, and then showed a downward trend. β-diversity analysis indicated that different nitrogen and phosphorus combinations significantly affected bacterial community structure. RDA analysis showed that soil available phosphorus and total nitrogen contributed the most to the difference of bacterial community structure. With the increase of nitrogen and phosphorus application amount, urease activity increased gradually, while alkaline phosphatase and sucrase activity displayed a trend of decrease. Therefore, excessive application of chemical fertilizer was not conducive to the maintenance and improvement of soil bacterial diversity and soil enzyme activity. |
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