| Cloning,structure and function analysis of CeDREB2.1 genein Cyperus esculentus |
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| DOI：10.7606/j.issn.1000-7601.2022.03.02 |
| Key Words: Cyperus esculentus DREB transcription factor gene cloning drought stress expression pattern expression pattern |
| Author Name | Affiliation | | CHAI Guaiqiang | College of Life Science,Yulin University, Yulin, Shaanxi 719000, China
Shaanxi Key Laboratory of Ecological Restoration in Shanbei Mining Area, Yulin, Shaanxi 719000, China | | QIN Yuanyuan | College of Life Science,Yulin University, Yulin, Shaanxi 719000, China | | KANG Yuxin | College of Life Science,Yulin University, Yulin, Shaanxi 719000, China | | HUANG Fan | College of Life Science,Yulin University, Yulin, Shaanxi 719000, China | | DUAN Yizhong | College of Life Science,Yulin University, Yulin, Shaanxi 719000, China
Shaanxi Key Laboratory of Ecological Restoration in Shanbei Mining Area, Yulin, Shaanxi 719000, China | | KANG Furen | College of Life Science,Yulin University, Yulin, Shaanxi 719000, China
Shaanxi Key Laboratory of Ecological Restoration in Shanbei Mining Area, Yulin, Shaanxi 719000, China |
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| Abstract: |
| Drought response element binding protein (DREB) can activate the expression of stress related genes and improve crop resistance. Cyperus esculentus plays an important role in drought resistance, water saving and ecological protection due to its drought tolerance and barren resistance. In this study, based on the previous transcription alsequencing, a DREB\|related transcription factor was found, and its function was preliminarily analyzed by PCR amplification, bioinformatics analysis, expression vector construction and abiotic stress expression. The results showed that a CeDREB2.1 (accession number: MZ713253) had an open reading frame of 1191bp and encoding 397 amino acids. Bioinformatics analysis showed that CeDREB2.1 had a typical AP2/ERFBP domain and was a hydrophilic protein. The proportion of α helix, extended chain, β fold and random curl were 26.01%, 6.57%, 2.78% and 64.65%, respectively. Phylogenetic analysis showed that CeDREB2.1 had high sequence similarity with DREB of other species, and it was closely related to DREB proteins of Populus euphrila, poplar and apple. Meanwhile, CeDREB2.1 was connected to pCSXN, and the plant over expression vector was successfully constructed,genetic transformation in Arabidopsis indicated CeDREB2.1 has the biological function of drought resistance. Furthermore,expression analysis in Cyperus esculentus showed that CeDREB2.1 could be induced by abiotic stresses such as drought, high temperature, low temperature, salt and ABA. This study provides theoretical reference for further explaining the biological function of CeDREB2.1 and mechanism of action involved in stress response. |
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