| Effects of drought stress on physiological characteristics of flag leaves and expression characteristics of drought\|resistant genes in roots of spring wheat |
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| DOI：10.7606/j.issn.1000-7601.2022.03.06 |
| Key Words: spring wheat drought stress photosynthetic characteristics physiological indicators gene expression |
| Author Name | Affiliation | | FANG Jing | School of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia 010020, China
Inner Mongolia Key Laboratory of Degradation Farmland Ecological Restoration and Pollution Control, Hohhot, Inner Mongolia 010031, China | | SHI Gongfu | School of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia 010020, China
Inner Mongolia Key Laboratory of Degradation Farmland Ecological Restoration and Pollution Control, Hohhot, Inner Mongolia 010031, China | | WEI Shuli | School of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia 010020, China
Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot, Inner Mongolia 010031, China
Inner Mongolia Key Laboratory of Degradation Farmland Ecological Restoration and Pollution Control, Hohhot, Inner Mongolia 010031, China | | CHENG Yuchen | Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot, Inner Mongolia 010031, China
Inner Mongolia Key Laboratory of Degradation Farmland Ecological Restoration and Pollution Control, Hohhot, Inner Mongolia 010031, China | | ZHANG Xiangqian | Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot, Inner Mongolia 010031, China
Inner Mongolia Key Laboratory of Degradation Farmland Ecological Restoration and Pollution Control, Hohhot, Inner Mongolia 010031, China | | WANG Jianguo | Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot, Inner Mongolia 010031, China
Inner Mongolia Key Laboratory of Degradation Farmland Ecological Restoration and Pollution Control, Hohhot, Inner Mongolia 010031, China | | AN Yu | School of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia 010020, China
Inner Mongolia Key Laboratory of Degradation Farmland Ecological Restoration and Pollution Control, Hohhot, Inner Mongolia 010031, China | | ZHAO Xiaoqing | School of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia 010020, China
Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot, Inner Mongolia 010031, China
Inner Mongolia Key Laboratory of Degradation Farmland Ecological Restoration and Pollution Control, Hohhot, Inner Mongolia 010031, China | | LU Zhanyuan | School of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia 010020, China
Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot, Inner Mongolia 010031, China
Inner Mongolia Key Laboratory of Degradation Farmland Ecological Restoration and Pollution Control, Hohhot, Inner Mongolia 010031, China |
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| Abstract: |
| The study on the effects of drought stress on the physiological characteristics of flag leaves and the expression of drought\|resistant genes in roots of spring wheat varieties is of great significance for stress\|resistant cultivation and new variety breeding of spring wheat. In this study, spring wheat varieties in the drought\|resistant group and the water\|sensitive group were used as test materials in the dry farming area at the western foot of Daxinganling. Two kinds of water control treatments were carried out: drought (rainproof in the dry shed) and control (regulation and replenishment of water). The differences of flag leaf photosynthetic indexes, physiological indexes, and root drought resistance gene expression of different varieties of spring wheat under drought stress were systematically analyzed. The results showed that there were significant differences in flag leaf photosynthetic characteristics, physiological characteristics, and root drought resistance gene expression between drought and control treatments (P<0.05). Under drought stress, the relative chlorophyll content, net photosynthetic rate (Pn), transpiration rate (Tr), and stomatal conductance (Gs<.sub>) of spring wheat flag leaves decreased significantly, and the decline range of drought\|resistant group was significantly lower than that of the water sensitive group. The physiological indexes POD, SOD, and Pro increased significantly, while MDA decreased significantly. Of which, the content of Pro in the drought resistance group was 90.36 μg·g-1, significantly higher than that in the water\|sensitive group (70.36 μmol·g-1), but the MDA content (19.82 μmol·g-1) was significantly lower than that in the water\|sensitive group (22.05 μmol·g-1). TaXTH-7A, TaWlip19, TaWdreb2 and TaBADHb gene were highly expressed in drought\|resistant group, and lowly expressed in water\|sensitive group. The results of correlation analysis showed that relative chlorophyll content, Tr, Pn, and Gs were significantly positively correlated with dry weight while intercellular carbon dioxide concentration (Ci), SOD, and MDA were significantly negatively correlated with it. In brief, under drought stress, drought resistance group spring wheat adjusted up the expression of root antioxidant enzyme genes and osmotic regulation gene automatically to raise the flag leaf POD and SOD activity of antioxidant enzymes, increase the content of Pro and other water\|soluble substances in the cytoplasm, reduce the concentration of intracellular reactive oxygen species, decrease its toxic effect on cells, and help spring wheat maintain a relatively high photosynthetic rate. Thus, the resistance to drought stress was enhanced. |
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