| Physiological response of 60Co- γ radiation alfalfa differentiated seedlings to heat stress |
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| DOI：10.7606/j.issn.1000-7601.2022.03.08 |
| Key Words: 60Co-γ radiation alfalfa differentiation seedlings heat stress physiological response membership function method |
| Author Name | Affiliation | | LI Bo | College of Agriculture, Forestry and Life Sciences, Qiqihar University, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar, Heilongjiang 161006, China | | LIU Chang | College of Agriculture, Forestry and Life Sciences, Qiqihar University, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar, Heilongjiang 161006, China | | FENG Chenglong | College of Agriculture, Forestry and Life Sciences, Qiqihar University, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar, Heilongjiang 161006, China | | LI Shanshan | College of Agriculture, Forestry and Life Sciences, Qiqihar University, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar, Heilongjiang 161006, China | | JIN Shanshan | College of Agriculture, Forestry and Life Sciences, Qiqihar University, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar, Heilongjiang 161006, China | | ZHAO Jinnuo | College of Agriculture, Forestry and Life Sciences, Qiqihar University, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar, Heilongjiang 161006, China | | LIU Jiayou | College of Agriculture, Forestry and Life Sciences, Qiqihar University, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar, Heilongjiang 161006, China | | ZHANG Junhui | College of Agriculture, Forestry and Life Sciences, Qiqihar University, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar, Heilongjiang 161006, China | | JIANG Siqi | College of Agriculture, Forestry and Life Sciences, Qiqihar University, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar, Heilongjiang 161006, China | | SHI Wanying | College of Agriculture, Forestry and Life Sciences, Qiqihar University, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar, Heilongjiang 161006, China |
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| Abstract: |
| To study the physiological response of alfalfa (Medicago sativa L.) under radiation and heat stress, the differentiated seedlings of alfalfa callus were used as materials. The treatments G3 and G4 of second generation tall plants were differentiation of leaf callus of irradiated alfalfa seed of 800 Gy and 600 Gy variant plants and CK was non irradiated alfalfa seed plants. The differentiated seedlings were subjected to high temperature stress at 40 ℃ for 1, 2, 3 and 4 days to investigate the survival rate and physiological and biochemical characteristics of alfalfa differentiation seedlings. The membership function and comprehensive D value of 8 indexes were calculated to comprehensively analyze the heat resistance of three groups of differentiated seedlings. The experimental results indicated that the survival rate of control differentiated seedlings was 64% under heat stress at 40℃ for 4 days. The survival rate of differentiated seedlings was seriously affected when the stress temperature was higher than 40℃ for more than 4 days. The effective simulated heat stress was 40℃ for 4 days. Under 40℃ heat stress, the contents of soluble protein, soluble sugar, proline and the activities of peroxidase and superoxide dismutase in three groups of differentiated seedlings increased first and then decreased with the increase of stress time, the activity of catalase decreased, and the content of malondialdehyde and relative conductivity increased. Under high temperature stress for 3 days，the contents of soluble protein, soluble sugar, proline, malondialdehyde, relative conductivity, peroxidase, and superoxide dismutase activities of G3 differentiated seedlings increased by 235.74%，79.21%，323.65%，30.10%，18.09%，82.87% and 350.91% respectively, and the catalase activity decreased by 64.52%. The comprehensive D value analysis of 8 physiological and biochemical indexes showed that the heat resistance of the three groups of differentiated seedlings was G3 > G4 > CK. A certain dose of radiation treatment improved the stress resistance of alfalfa differentiated seedlings. |
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