Effects of green manure and nitrogen application on soil enzyme activities in a dryland winter wheat field
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DOI:10.7606/j.issn.1000-7601.2022.03.16
Key Words: soil enzyme activity  green manure  nitrogen level  total enzyme activity
Author NameAffiliation
FENG Xiaoling College of Urban and Environment Science,Northwest University, Xi’an, Shaanxi 710127, China 
WANG Jun College of Urban and Environment Science,Northwest University, Xi’an, Shaanxi 710127, China
Shaanxi Key Laboratory of Earth Surface System and Environmental Carrying Capacity, Xi’an, Shaanxi 710127, China 
GAO Yuan College of Urban and Environment Science,Northwest University, Xi’an, Shaanxi 710127, China 
WEN Mengmeng College of Urban and Environment Science,Northwest University, Xi’an, Shaanxi 710127, China 
DOU Ying College of Urban and Environment Science,Northwest University, Xi’an, Shaanxi 710127, China 
FANG Zhenwen College of Urban and Environment Science,Northwest University, Xi’an, Shaanxi 710127, China 
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Abstract:
      Based on a winter wheat-green manure rotation field experiment, the responses of soil enzyme activities to green manure and nitrogen application were studied. Four green manures as Changwu soyean, Sudan grass, Changwu soyean+ Sudan grass mixed and fallow control were adopted during summer fallow period, and nitrogen was applied to winter wheat at rates of 0, 60, 120 kg·hm-2. The activities of β-1,4-glucosidase,cellobiohydrolase, β-1,4-N-acetylglucosaminidase, and leucine aminopeptidase were measured. Compared with the control, planting Changwu soyean, Sudan grass, and mixture of Changwu soyean+Sudan grass during the fallow period significantly increased soil enzymes activities by 12.9%~59.2%, enzyme activity increased by 12.9%~59.3% and 6.19%~48.8% at 0~10 cm and 10~20 cm. No significant differences between green manure treatments were found for soil β-glucosidase and cellobiohydrolase activities. Planting Changwu soyean significantly increased acetylglucosaminidase and leucine aminopeptidase activities by 9.9%~26.2% compared with planting Sudan grass and mixture of Changwu soyean and Sudan grass. The activities of four soil enzymes all increased with the increases of nitrogen application rates. Nitrogen fertilizer and green manure had an interactive effect on cellobiohydrolase activity. In the dry farming system of the Loess Plateau, green manure should be included during summer fallow period to stimulate soil enzyme activities and enhance soil fertilities.