| Effects of exogenous 5-ALA on growth of maize seedlings and expression of antioxidant enzyme genes under drought stress |
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| DOI：10.7606/j.issn.1000-7601.2022.04.01 |
| Key Words: maize drought stress 5-aminolevulinic acid growth of maize seedling antioxidant enzyme gene expression |
| Author Name | Affiliation | | WANG Peng | College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | YANG Aojun | College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | FENG Zhijin | College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | WANG Ting | College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | JIN Zihao | College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | WANG Fang | College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | PENG Yunling | College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China |
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| Abstract: |
| In this study, drought\|resistant maize inbred line Zheng 58 and drought\|sensitive maize inbred line TS141 were used as materials to study the alleviating effect of 5-aminolevulinic acid (5-ALA) on the effect of maize seedling growth and antioxidant enzyme gene expression under 15% PEG-6000 simulated drought stress. The results showed that compared with the control, the seedling length, fresh weight and dry weight of maize inbred line Zheng 58 and drought sensitive TS141 decreased obviously under drought stress. Zheng 58 decreased by 18.49%, 29.06% and 20.00% respectively, and TS141 decreased by 25.66%, 23.97% and 13.64% respectively. The contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2) in leaves increased significantly. The content of MDA increased by 164.58% and 263.53% respectively, and the content of H2O2 increased by 134.95% and 203.83% respectively. The activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbic acid peroxidase (APX) increased, and Zheng 58 increased by 65.61%, 29.48%, 68.49% and 141.51% respectively, and TS141 increased by 63.01%, 48.00% and 63.01% respectively. Chlorophyll content decreased by 33.46% and 42.26% respectively. Pn, Gs and Tr of Zheng 58 decreased by 48.78%, 45.31% and 52.39% respectively, while those of TS141 decreased by 57.66%, 57.46% and 65.14% respectively. The relative expression of antioxidant enzymes SOD3, POD3 and CAT1 increased, and Zheng 58 increased by 54.58%, 34.12% and 69.70% respectively, and TS141 increased by 54.95%, 21.36% and 59.34% respectively. Spraying 25 mg·L-1 5-ALA obviously alleviated the damage of maize seedlings caused by drought stress. Compared with PEG treatment, the seedling length, fresh weight and dry weight of maize inbred lines Zheng 58 and TS141 increased significantly, with the seedling length increased by 13.31% and 11.21%, the fresh weight increased by 22.29% and 18.23%, and the dry weight increased by 16.67 respectively. In leaves, MDA decreased by 17.85%, 25.39% and H2O2 decreased by 23.26%, 19.15% respectively. The activities of SOD, POD, CAT and APX increased further, Zheng 58 increased by 16.49%, 18.15%, 36.98% and 28.13% respectively, and TS141 increased by 16.65%, 16.54%, 28.49% and 26.76% respectively. Chlorophyll content and photosynthetic parameters Pn, Gs and Tr increased, and chlorophyll content increased by 18.29% and 19.87% respectively. Photosynthetic parameters Pn increased by 35.87% and 42.12%, Gs by 36.63% and 36.81%, and Tr by 37.67% and 67.88%, respectively. Compared with drought stress treatment, the relative expression levels of antioxidant enzyme genes SOD3, POD3 and CAT1 in maize leaves were further increased, Zheng 58 increased by 20.99%, 32.18% and 23.51% and TS141 increased by 25.97%, 23.16% and 40.94% respectively. |
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