| Control efficiency and root physiological response of cucumber grafted seedlings and cucumber straight root seedlings inoculated with Trichoderma harzianum on root rot |
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| DOI：10.7606/j.issn.1000-7601.2022.04.02 |
| Key Words: cucumber seedling grafting cucumber seedling root rot Trichoderma harzianum |
| Author Name | Affiliation | | JIN Yazhong | College of Horticulture and Landscape Architecture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | XIONG Yanan | College of Horticulture and Landscape Architecture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | QI juan | College of Horticulture and Landscape Architecture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | LIU Leiqing | Golden Ma Ma Agricultural Science & Technology Company, Qingdao, Shandong 266000, China | | REN Jinli | College of Horticulture and Landscape Architecture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | LI Chunxia | College of Horticulture and Landscape Architecture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | LU Meiyan | College of Horticulture and Landscape Architecture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | CHEN Hui | College of Horticulture and Landscape Architecture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | CHEN Yongchi | College of Horticulture and Landscape Architecture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China |
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| Abstract: |
| To explore the different ability between grafting culture and Trichoderma harzianum in improving the resistance of cucumber seedlings to root rot and its physiological regulation, cucumber straight root seedlings were treated with the spore suspensions of Trichoderma harzianum DQ002 and root rot pathogen by inoculation treatment in root, and grafted seedlings were treated with root rot pathogen by inoculation treatment in root. Meanwhile, the incidence rate of root rot and physiological changes in roots of cucumber straight root seedlings and grafted seedlings were measured. The results showed that Trichoderma harzianum DQ002 inhibited the accumulation of H2O2 and the production rate of O-2 by stimulating the activities of POD, PPO, SOD in the roots of cucumber straight seedlings. The activities of PAL, chitinase, β-1, 3-glucanase and the flavonoid content in root of cucumber straight root seedlings were increased, and the disease resistance was improved. Under the condition of inoculation with root rot pathogen, grafting (T2) increased POD activity, PPO activity in the early stage and SOD activity in the late stage, and decreased H2O2 accumulation and O-2 production rate, and promoted the activities of PAL and chitinase and β-1, 3-glucanase activity in the late stage. However, H2O2 accumulation and O-2 production rate in cucumber grafted seedlings were significantly higher than those in the straight root seedlings treated with Trichoderma harzianum DQ002(including T3 and T4), while the activities of PAL, chitinase, β-1, 3-glucanase and flavonoid content were markedly lower than those in the straight root seedlings treated with Trichoderma harzianum DQ002. In addition, the incidence and index of T3 (application of pathogen 2 days prior to application of Trichoderma) and T4 (application of Trichoderma 2 days prior to application of pathogen) were 22.39% and 17.87%, and disease index was 23.03% and 14.33%, respectively, which was significantly lower than that of CK1 (application of water), T1 (only application of pathogen) and cucumber grafted seedlings (T2). However, the application of Trichoderma harzianum in different times had different control effects on disease. The results indicated that both grafting culture of cucumber and Trichoderma harzianum DQ002 treatment on cucumber straight root seedlings improved the resistance of cucumber seedlings to root rot, but there were differences in the regulation of root physiology between both treatments, which might be one of the reasons for the difference in the occurrence of root rot. |
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