| Effects of calcium fertilizer application on peanut rhizosphere bacterial community structure under salt stress |
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| DOI：10.7606/j.issn.1000-7601.2022.04.05 |
| Key Words: peanut salt stress rhizosphere soil soil microbial community 16S rRNA |
| Author Name | Affiliation | | DAI Liangxiang | Shandong Peanut Research Institute，Qingdao, Shandong 266100, China | | DING Hong | Shandong Peanut Research Institute，Qingdao, Shandong 266100, China | | XU Yang | Shandong Peanut Research Institute，Qingdao, Shandong 266100, China | | ZHANG Guanchu | Shandong Peanut Research Institute，Qingdao, Shandong 266100, China | | SHI Xiaolong | College of Agronomy，Shenyang Agricultural University，Shenyang, Liaoning 110866，China | | QIN Feifei | Shandong Peanut Research Institute，Qingdao, Shandong 266100, China | | GUO Qing | Shandong Peanut Research Institute，Qingdao, Shandong 266100, China | | JIANG Changsong | Haiyang Fruit Industry Development Service Center, Yantai, Shandong 265100, China | | ZHANG Zhimeng | Shandong Peanut Research Institute，Qingdao, Shandong 266100, China |
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| Abstract: |
| To assess the peanut rhizosphere bacteria community structure in response to the basal application of calcium fertilizer under salt stress at different development stages and enhance plant stress tolerance by improving the rhizosphere microbial environment of saline\|alkali soil, a pot experiment was set up with different salt concentrations. The rhizosphere soils of different growth stages were used to extract DNA for constructing bacterial 16S rRNA gene library, and then high\|throughput sequencing was performed for sequencing and bioinformatics analysis. The results showed that (1) the microbial community diversity of the soil samples was high and evenly distributed, and salt stress increased the richness and diversity of the microbial community in the rhizosphere, which was associated with the growth period. (2) Taxonomic analysis exhibited that the dominant classes and families were identical in all the soil groups: the dominant classes were Actinobacteria, Alphaproteobacteria, Gammaproteobacteria, Verrucomicrobiae, Saccharimonadia, Gemmatimonadetes, Bacteroidia and Acidobacteriia, and the dominant families were norank_o__Saccharimonadales, Sphingomonadaceae, Gemmatimonadaceae, Burkholderiaceae, norank_o__Gaiellales. (3) The abundance of Gammaproteobacteria and Verrucomicrobiae were significantly increased after salt stress treatment and basal calcium fertilizer application, and the richness improved more obviously with the prolongation of the growth period and the increase of salt concentration. The abundance of the two fungi was 1.2, 1.8 times and 1.6, 1.5 times higher than that of the control under high salt stress, respectively. However, basal calcium fertilizer application under salt stress reduced the abundance of Actinobacteria and Saccharimonadia, which decreased significantly with increasing salt concentration. Moreover, the decrease rate of the two fungi were larger at the flowering and needling stage. Chitinophagaceae and Opitutaceae were evidently affected by salt concentration and growth stages and increased dramatically with the increase of salt concentration. (4) The sequences related to amino acid metabolism, energy metabolism, cofactor and vitamin metabolism, and nucleotide metabolism were significantly enriched after basal calcium fertilizer treatment at the fast\|growing stage. (5) Salt stress seriously inhibited the development of peanut kernel and reduced the yield. High salt stress reduced pod yield by 40.4%~50.0%. |
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