| Phylogenetic evolution of NAC gene families in four poaceae species and expression profile analysis of maize ZmNACs under drought stress |
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| DOI：10.7606/j.issn.1000-7601.2022.04.26 |
| Key Words: poaceae species phylogenetic analysis NAC genes drought stress maize gene expression |
| Author Name | Affiliation | | LI Lihua | Cereal Crop Research, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China | | GUO Yingbin | Jining Technical College, Jining, Henan 272100, China | | WEI Xin | Cereal Crop Research, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China |
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| Abstract: |
| In this research, we used bioinformatics methods to identify and analyze the NAC gene families in four poaceaes including Zea mays, Sorghum bicolor, Brachypodium distachyon and Oryza sativa. Evolutionary analysis showed that 556 NAC genes in these four poaceaes were clustered into six clades. NAC transcription factors in these four species had similar physicochemical properties but were different in clades. Clade 1, 2, 3 and clade 4, 5, 6 showed similar relative molecular weight, respectively, and clade 1, 2, 3, 5 showed similar isoelectric points, and so did in clade 4 and 6. A total of 27 NAC genes with transmembrane domains were identified in these four species, and most (88.9%) of them were in clade 4. A total of 174 poaceae ancestral genes were identified by the traceability method and differences existed in both the loss and expansion of NAC genes in each species. Duplication events analysis discovered that tandem duplication events contributed significantly to the expansion of NAC gene in poaceae (20.3%; 113/556), and segment duplication events in maize contributed significantly to the expansion of maize NAC genes (30.5%; 50/164). By transcriptional expression analysis, 51 differentially expressed genes were identified in maize under drought stress, and the number of differentially expressed genes in different tissues was different. The number of differentially expressed genes in leaves and female ears was more than that in male ears. This research provides references for further exploring the evolution of NAC genes in gramineous varieties and identifying functional NAC genes in maize. |
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