| Relationship between root morphology, physiological and biochemical characteristics of leaves and drought resistance of Chuanxiang 29B near\|isogenic introgression lines at seedling stage |
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| DOI：10.7606/j.issn.1000-7601.2022.04.27 |
| Key Words: rice near\|isogenic introgression lines drought resistance seedling stage root morphology physiological and biochemical characteristics |
| Author Name | Affiliation | | LI Qiyong | Institute of Plant Protection, Sichuan Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management on Crops in Southwest, Ministry of Agriculture, Chengdu, Sichuan 610066, China | | ZHU Conghua | Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 610066, China | | LI Xingyue | Institute of Plant Protection, Sichuan Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management on Crops in Southwest, Ministry of Agriculture, Chengdu, Sichuan 610066, China | | YI Jun | Institute of Plant Protection, Sichuan Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management on Crops in Southwest, Ministry of Agriculture, Chengdu, Sichuan 610066, China | | WU Binglin | Institute of Plant Protection, Sichuan Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management on Crops in Southwest, Ministry of Agriculture, Chengdu, Sichuan 610066, China | | FU Huijuan | Institute of Plant Protection, Sichuan Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management on Crops in Southwest, Ministry of Agriculture, Chengdu, Sichuan 610066, China | | ZHANG Hong | Institute of Plant Protection, Sichuan Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management on Crops in Southwest, Ministry of Agriculture, Chengdu, Sichuan 610066, China |
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| Abstract: |
| To reduce the genetic background difference of materials, rapidly identify rice drought resistance and screen identification indicators, the seedlings of Chuanxiang 29B near\|isogenic introgression lines and its recurrent parent Chuanxiang 29B were treated with the repeated drought stress in the greenhouse. The change of seedling matter, morphological indicators, root indicators and physiological biochemical indicators of leaves were measured to analyze the relations between each indicator and drought resistance and screen the identification indicators of drought resistance through correlation analysis and regression analysis. The results showed that root dry matter accumulation and root\|shoot ratio were significantly increased by 25.59% and 45.60% （P＜0.05） under the repeated drought stress at seedling stage. Average contents of carotenoid, malondialdehyde, abscisic acid and ethylene in leaves were significantly increased by 29.17%, 6.03%, 25.25%, 40.74% （P＜0.05）, respectively. The average content of soluble protein was increased by 9.77%. The enzymatic activity mean values of peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), pyrroline-5-carboxylate synthetase (P5CS), ornithine δ-aminotransferase (δ-OAT) and proline dehydrogenase (ProDH) in leaf were increased by 11.81%, 37.43%, 25.91%, 32.65%, 31.46%, 29.07%（P＜0.05）, respectively. On the contrary, the mean values of aboveground dry matter accumulation, leaf SPAD value, chlorophyll a content (Chl-a), chlorophyll b content (Chl-b), leaf auxin, leaf cytokinin, leaf gibberellin were decreased by 14.22%, 9.00%, 18.79%, 55.32%, 31.04%, 19.29%, 34.79%（P＜0.05）, respectively. The first drought survival rate, the second drought survival rate and the repeated drought survival rate of Chuanxiang 29B/ASOMINORI//29B///29B (C1) were 90.86%, 82.86% and 86.86%, respectively, all of which were the highest. The repeated drought survival rates of Chuanxiang 29B/ASOMINORI//29B///29B(C3) and Chuanxiang 29B/ASOMINORI//29B///29B////29B(C4) were 71.51% and 72.72%, respectively, which were significantly lower than those of the other four materials. The correlation analysis and stepwise regression showed that the relative value of root surface area, total root length, reduced glutathione (GSH) content and peroxidase activity in leaves could be used as identification indicators of drought resistance at rice seedling stage. The repeated drought survival rate at seedling stage could be directly used to identify drought resistance of rice. Based on the repeated drought survival rate, Chuanxiang 29B/ASOMINORI//29B///29B (C1) had the strongest drought resistance in tested materials at seedling stage. |
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