| Identification and expression analysis of BraSAUR gene family in rapeseed (Brassica rapa L.) |
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| DOI：10.7606/j.issn.1000-7601.2022.05.01 |
| Key Words: SAUR gene family Brassica rapa L. identification low temperature and drought stress expression pattern |
| Author Name | Affiliation | | PAN Qiaowen | College of Agronomy, Gansu Agricultural University, Gansu Oilseed rape Engineering Technology Research Center, Lanzhou, Gansu 730070, China | | WU Junyan | College of Agronomy, Gansu Agricultural University, Gansu Oilseed rape Engineering Technology Research Center, Lanzhou, Gansu 730070, China | | MA Xuecai | College of Agronomy, Gansu Agricultural University, Gansu Oilseed rape Engineering Technology Research Center, Lanzhou, Gansu 730070, China | | WANG Yalin | College of Agronomy, Gansu Agricultural University, Gansu Oilseed rape Engineering Technology Research Center, Lanzhou, Gansu 730070, China | | MA Li | State Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China | | XU Xinyu | College of Agronomy, Gansu Agricultural University, Gansu Oilseed rape Engineering Technology Research Center, Lanzhou, Gansu 730070, China | | LI Peng | College of Agronomy, Gansu Agricultural University, Gansu Oilseed rape Engineering Technology Research Center, Lanzhou, Gansu 730070, China | | YAO Yanlin | College of Agronomy, Gansu Agricultural University, Gansu Oilseed rape Engineering Technology Research Center, Lanzhou, Gansu 730070, China | | XU Fang | College of Agronomy, Gansu Agricultural University, Gansu Oilseed rape Engineering Technology Research Center, Lanzhou, Gansu 730070, China | | CUI Xiaoru | Gansu Agricultural Technology Extension Station, Lanzhou, Gansu 730070, China | | SUN Wancang | College of Agronomy, Gansu Agricultural University, Gansu Oilseed rape Engineering Technology Research Center, Lanzhou, Gansu 730070, China |
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| Abstract: |
| SAUR (Small auxin\|up RNA) is an early auxin response gene to stress. In this paper, we studied the information of SAUR family in the whole rapeseed genome (Brassica rapa L.) and analyzed the basic characteristics of the family members. Longyou 7 (L7) and Longlenox (X) were used as experimental materials with low temperature and drought stress treatments. The expression levels of different genes in different varieties were analyzed by fluorescence quantitative technique to provide support for the study of auxin early response gene regulation mechanism of winter rapeseed growing point. Results showed that a total of 142 SAUR genes were systematically identified from Brassica rapa, which were distributed on 10 chromosomes, mainly on chromosome 2 and 3 and divided into four groups according to the evolutionary tree relationship. The genes were short in length and most had no introns. The results of qRT-PCR showed that after low temperature treatment, the expression of Bra029452 in the leaves of two varieties had different trends compared with CK, and the expression of Bra010501 in the leaves of L7 was 24 times more than that of X at 24 h. The expression of Bra010501 in growing point of L7 was 4 times more than that of CK at 24 h, while in X was first increased and then decreased. Under low temperature stress and drought stress, Bra029452 gene increased gradually in the leaves and growing points of L7, suggesting that Bra029452 gene was involved in regulation of low temperature and drought tolerance of Brassica rapa. |
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