| Effects of potash fertilizer types and rates on alfalfa root crown saccharides content and cold resistance under low temperature stress |
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| DOI：10.7606/j.issn.1000-7601.2022.05.07 |
| Key Words: alfalfa root crown types of potash fertilizer saccharides cold resistance |
| Author Name | Affiliation | | SUN Mingxue | College of Agriculture, Inner Mongolia Minzu University, Inner Mongolia Autonomous Region Feedstuff Engineering Technology Research Center, Tongliao, Inner Mongolia 028041, China | | ZHANG Yuxia | College of Agriculture, Inner Mongolia Minzu University, Inner Mongolia Autonomous Region Feedstuff Engineering Technology Research Center, Tongliao, Inner Mongolia 028041, China | | XIA Quanchao | College of Agriculture, Inner Mongolia Minzu University, Inner Mongolia Autonomous Region Feedstuff Engineering Technology Research Center, Tongliao, Inner Mongolia 028041, China | | WANG Xianguo | College of Grassland Science and Technology, China Agricultural University, Beijing 028000, China | | ZHANG Qingxin | College of Agriculture, Inner Mongolia Minzu University, Inner Mongolia Autonomous Region Feedstuff Engineering Technology Research Center, Tongliao, Inner Mongolia 028041, China | | LIU Tingyu | College of Agriculture, Inner Mongolia Minzu University, Inner Mongolia Autonomous Region Feedstuff Engineering Technology Research Center, Tongliao, Inner Mongolia 028041, China | | ZHANG Yongliang | College of Agriculture, Inner Mongolia Minzu University, Inner Mongolia Autonomous Region Feedstuff Engineering Technology Research Center, Tongliao, Inner Mongolia 028041, China |
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| Abstract: |
| This study applied 3 types of KCl, K2SO4 and KH2PO4 potash fertilizers to alfalfa （M.sativa ‘Gibraltar’） to explore the effects of potash fertilizer types and dosages on cold resistance of alfalfa overwintering organs and the physiological mechanism of carbohydrate protection. Three application rates including 100 kg·hm-2K2O, 200 kg·hm-2K2O and 300 kg·hm-2K2O (indicated by K1, K2 and K3, respectively), and the control (CK) group without potash fertilizer were used. The overwintering organs of alfalfa were excavated in the pre\|freezing period to simulate low temperature freezing stress (4℃,-10℃,-20℃ and -30℃), and the root crown vitality, soluble sugar, sucrose, fructose and starch content were measured. With the decrease of low temperature and freezing stress, the root crown vitality and starch content of alfalfa treated with KCl, K2SO4 and KH2PO4 showed a continuous decreasing trend, with minimum value reaching at -30℃. The root crown activity and starch content of alfalfa treated at 4℃ were significantly different, which were 86.25%～91.59% and 45.47%～66.07% lower than those treated at 4℃, respectively. The content of soluble sugar, sucrose and fructose showed a trend of first increasing and then decreasing, and all reached the maximum at -20℃, and they were 431.00～513.93 mg·g-1, 235.23～329.05 mg·g-1 and 185.75～243.79 μg·g-1, respectively. With the increase of the amount of potash fertilizer, the root crown vitality, soluble sugar, sucrose, fructose, and starch content of alfalfa treated with 3 potash fertilizers of KCl, K2SO4, and KH2PO4 showed a trend of first increasing and then decreasing, and under different low temperature stress treatments, the effect of K2SO4 treatment on alfalfa root crown vitality, soluble sugar, sucrose, fructose, and starch content was the most obvious. When the application rate was 200 kg·hm-2 K2O, the alfalfa root crown vitality, soluble sugar, sucrose, fructose, and starch content were significantly different from CK (P＜0.05), and compared with CK, it increased by 56.03%～197.26%, 16.38%～48.77%, 24.13%～46.22%, 30.53%～59.95% and 35.73%～67.87%, respectively. It indicated that the application of K2SO4 with a dosage of 200 kg·hm-2K2O was more conducive to regulating the osmotic potential of alfalfa root crown cells, lowering the freezing point of cytoplasm, protecting biofilm, and improving the cold resistance of alfalfa overwintering organs. |
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