| Effects of drought stress on physiology characteristics of Radix paeoniae Rubra and clonal expression of related genes |
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| DOI：10.7606/j.issn.1000-7601.2022.05.08 |
| Key Words: Radix paeoniae Rubra drought stress resistance physiology DREB gene clone gene expression level |
| Author Name | Affiliation | | WANG Dandan | College of Agriculture, Eastern Liaoning University, Dandong, Liaoning 118000, China | | WANG Peng | College of Agriculture, Eastern Liaoning University, Dandong, Liaoning 118000, China | | HAN Zhiyang | College of Agriculture, Eastern Liaoning University, Dandong, Liaoning 118000, China | | LIU Danmei | College of Agriculture, Eastern Liaoning University, Dandong, Liaoning 118000, China |
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| Abstract: |
| This study used potted water control method to explore the effects of different drought stresses on growth and drought resistance mechanism of Radix paeoniae. Three treatments of control, mild drought, and severs drought were used to test the leaf relative water content, soluble sugar, soluble protein, net photosynthetic rate, transpiration rate, stomatal conductance, intracellular CO2 concentration, ascorbic acid, chlorophyll, carotenoids, flavonoids, maltaraldehyde, free proline, and superoxidase dismutase to evaluate the drought resistance of Radix paeoniae. Six drought\|related genes (DREB、Psb27、PsaK、MDHAR、SOD and GR) were cloned by RT-PCR with RACE to explore the effects of different drought levels on gene expression levels of Radix paeoniae. The results showed that, under severe drought stress, the soluble sugar, soluble protein, carotenoids, flavonoids, proline, maltaraldehyde, ascorbic acid and glutathione all increased, for example, after 24 d of severe and mild drought, the soluble sugar content of Radix paeoniae was about 2.50 times and 1.66 times that of the control group, respectively. After 16 day of severe drought, the soluble protein content was about 2.43 times that of the control group, and the contents of carotenoid and flavonoid were separately about 1.8 times and 1.4 times that of the control group, and about 1.4 times and 1.2 times that of mild drought; while the leaf relative water content, the transpiration rate, stomatal conductivity, net photosynthetic rate and chlorophyll contentall decreased.For example, the leaf relative water content of Radix paeoniae was less than 20% under severe drought stress, while both mild drought and control groups were above 55%, and the chlorophyll content fell to the lowest value at the 16th day, about 26% of the control group and 33% of mild drought. Under severe drought stress, the expression of photosynthesis associated gene Psb27, PsaK was upregulated, and the severe drought upregulated level was higher than mild drought and control. The expression of most of antioxidant genes (SOD,GR), ABA content, and intracellular CO2 concentration all increased first then decreased, such as SOD expression levels peaked at the 16th day under severe drought, about 1.58 times and 1.86 times in the mild drought and the control group, respectively, and then the expression level decreased to 1.43 times by the 24th day of the control group. The expression level of MDHAR decreased, and at the 24th day under severe drought, its expression level decreased to 5.53 times that of the control group. |
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