| Cloning and expression analysis of buckwheat FeHSP83 gene under drought and salt stress |
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| DOI：10.7606/j.issn.1000-7601.2022.05.10 |
| Key Words: sweet buckwheat FeHSP83 gene clone adversity expression mode |
| Author Name | Affiliation | | XU Rui | College of Agriculture, Yangtze University, Hubei Center for Collaborative Innovation of Grain Industry, Jingzhou, Hubei 434025, China | | XIONG Zehao | College of Agriculture, Yangtze University, Hubei Center for Collaborative Innovation of Grain Industry, Jingzhou, Hubei 434025, China | | ZHU Xudong | College of Agriculture, Yangtze University, Hubei Center for Collaborative Innovation of Grain Industry, Jingzhou, Hubei 434025, China | | ZHOU Binhan | College of Agriculture, Yangtze University, Hubei Center for Collaborative Innovation of Grain Industry, Jingzhou, Hubei 434025, China | | HOU Zehao | College of Agriculture, Yangtze University, Hubei Center for Collaborative Innovation of Grain Industry, Jingzhou, Hubei 434025, China | | XU Jiasheng | College of Agriculture, Yangtze University, Hubei Center for Collaborative Innovation of Grain Industry, Jingzhou, Hubei 434025, China | | LUO Yirou | College of Agriculture, Yangtze University, Hubei Center for Collaborative Innovation of Grain Industry, Jingzhou, Hubei 434025, China | | FANG Zhengwu | College of Agriculture, Yangtze University, Hubei Center for Collaborative Innovation of Grain Industry, Jingzhou, Hubei 434025, China |
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| Abstract: |
| This study used ‘Xinong 9976’ as the experimental material to explore the expression pattern of the FeHSP83 gene of sweet buckwheat under drought and salt stress. The buckwheat seedlings were subjected to stress treatment with 15% PEG 6000 solution and 0.1% NaCl solution. Through the RT-PCR method, FeHSP83 was identified. The gene had an open reading frame of 2 100 bp and encoded 704 amino acids. Its relative molecular mass was predicted to be 80.97 kDa, and the theoretical isoelectric point was 4.96. It had strong hydrophilicity. The result of homology analysis showed that the gene was closely related to quinoa and sugar beet, and the homology reached 97.06%. The qRT-PCR method was used to analyze the expression level of FeHSP83 gene in leaves and roots under drought and salt stress. The results showed that under drought stress, the expression of FeHSP83 in the roots of buckwheat continued to increase, reaching the maximum at 48 h, with an increase in the range of 1.03~10.20; the expression of leaves showed a trend of increasing first and then decreasing; while under salt stress conditions FeHSP83 had a more obvious response in the leaves of plants, and the increase range was 1.07~6.24, and the expression reached the highest at 12 h; the expression of roots increased rapidly at 3 h, and then slowly decreased. The results showed that FeHSP83 gene quickly responded to stress under drought and salt stress, and the expression of FeHSP83 gene in different organs of roots and leaves was different. Therefore, FeHSP83 gene played a certain role in drought resistance of sweet buckwheat. |
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