| Cold tolerance evaluation and genetic diversity analysis of winter Brassica napus |
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| DOI：10.7606/j.issn.1000-7601.2022.06.01 |
| Key Words: winter Brassica napus cold resistance molecular marker genetic diversity |
| Author Name | Affiliation | | MA Min | College of Agronomy, Gansu Agricultural University/Gansu Province Rape Engineering Technology Research Center/Gansu Provincial Key Laboratory of Aridland Crop Science/Gansu Key Laboratory of Crop Improvement & GermplasmEnhancement, Lanzhou, Gansu 730070, China | | ZHU Mingchuan | College of Agronomy, Gansu Agricultural University/Gansu Province Rape Engineering Technology Research Center/Gansu Provincial Key Laboratory of Aridland Crop Science/Gansu Key Laboratory of Crop Improvement & GermplasmEnhancement, Lanzhou, Gansu 730070, China | | ZENG Rui | College of Agronomy, Gansu Agricultural University/Gansu Province Rape Engineering Technology Research Center/Gansu Provincial Key Laboratory of Aridland Crop Science/Gansu Key Laboratory of Crop Improvement & GermplasmEnhancement, Lanzhou, Gansu 730070, China | | WU Junyan | College of Agronomy, Gansu Agricultural University/Gansu Province Rape Engineering Technology Research Center/Gansu Provincial Key Laboratory of Aridland Crop Science/Gansu Key Laboratory of Crop Improvement & GermplasmEnhancement, Lanzhou, Gansu 730070, China | | MA Li | College of Agronomy, Gansu Agricultural University/Gansu Province Rape Engineering Technology Research Center/Gansu Provincial Key Laboratory of Aridland Crop Science/Gansu Key Laboratory of Crop Improvement & GermplasmEnhancement, Lanzhou, Gansu 730070, China | | LI Xuecai | College of Agronomy, Gansu Agricultural University/Gansu Province Rape Engineering Technology Research Center/Gansu Provincial Key Laboratory of Aridland Crop Science/Gansu Key Laboratory of Crop Improvement & GermplasmEnhancement, Lanzhou, Gansu 730070, China | | PU Yuanyuan | College of Agronomy, Gansu Agricultural University/Gansu Province Rape Engineering Technology Research Center/Gansu Provincial Key Laboratory of Aridland Crop Science/Gansu Key Laboratory of Crop Improvement & GermplasmEnhancement, Lanzhou, Gansu 730070, China | | LIU Lijun | College of Agronomy, Gansu Agricultural University/Gansu Province Rape Engineering Technology Research Center/Gansu Provincial Key Laboratory of Aridland Crop Science/Gansu Key Laboratory of Crop Improvement & GermplasmEnhancement, Lanzhou, Gansu 730070, China | | ZENG Xiucun | HeXi University Zhangye, Gansu 734000, China | | ZHU Wenying | Agricultural Technology Extension Center, Kongtong District, Pingliang City, Gansu Province, Pingliang, Gansu 744099, China | | SUN Wancang | College of Agronomy, Gansu Agricultural University/Gansu Province Rape Engineering Technology Research Center/Gansu Provincial Key Laboratory of Aridland Crop Science/Gansu Key Laboratory of Crop Improvement & GermplasmEnhancement, Lanzhou, Gansu 730070, China |
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| Abstract: |
| In view of the differences in cold resistance and genetic diversity among different varieties of Brassica napus, a randomized block design was adopted in 2021. The differences in overwintering rate, vernalization characteristics and genetic diversity between 32 varieties of Brassica napus in Gansu Province and 9 varieties of Brassica napus in central Shaanxi and Jiangsu Province were analyzed. The systematic cluster analysis and SSR markers were used to explore the differences in cold resistance. The results showed that the overwintering rate of winter Brassica napus varieties in Gansu Province was higher than 80%, and the overwintering rate of winter Brassica napus varieties in central Shaanxi and Jiangsu areas were less than 25% with the significant differences in cold resistance. According to the vernalization rate, the tested materials were divided into four levels: strong winter, winter, semi winter and weak winter. The proportion of plants without buds in strong winter materials was high. It was difficult to bolt and bud into reproductive growth after spring sowing. The weighted average bolt height was low, which had strong cold resistance. The higher the vernalization rate, the higher the weighted average plant height at maturity and the weaker the resistance. The overwintering rate was negatively correlated with the vernalization rate. A total of 976 loci were amplified by 98 pairs of primer combinations with high polymorphism, of which 750 were polymorphic bands, and the ratio of polymorphic loci was 72%, indicating that the polymorphism richness at the molecular genetic level was high. The genetic diversity of winter Brassica napus in central Shaanxi and Jiangsu areas was higher than that of winter Brassica napus in Gansu Province. Through cluster analysis, winter Brassica napus were divided into 9 categories. According to the analysis of population genetic structure and principal component analysis, it was divided into three categories, which were consistent with the classification of cold resistance. The research results could lay a foundation for the further study of variety identification, fingerprint construction and selection of suitable varieties in different ecological areas of Brassica napus. |
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