| Comprehensive evaluation and screening of cold tolerance of 240 broomcorn millet germplasm resources at germination stage |
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| DOI：10.7606/j.issn.1000-7601.2022.06.03 |
| Key Words: broomcom millet bud stage cold resistance comprehensive evaluation |
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| Abstract: |
| Breeding of cold\|resistant varieties is the most direct and effective means to solve the problem of cold damage in agricultural production. In this study, 240 millet resources were used as test materials and 25℃ environment was considered as the control treatment. The effects of low temperature stress at 10℃ on the growth and development of broomcorn millet at germination stage were studied. The tolerance of different broomcorn cultivars to cold stress was evaluated by descriptive statistical analysis, correlation linear analysis, principal component analysis and cluster analysis. The results showed that under cold stress at 10℃, the average values of each index at germination stage of 240 broomcorn millet resources were lower than those of the control group, but there were significant differences among different varieties. The variation coefficients of each index ranged from 20.59% to 100.03%, indicating that the broomcorn millet resources tested had rich genetic diversity. Correlation analysis showed that the relative germination potential, relative germination index and relative germination rate were significantly positively correlated, and the relative bud fresh weight, relative vigor index and relative root fresh weight were significantly positively correlated, indicating that these cold tolerance traits had a great degree of correlation. The results of principal component analysis showed that the cumulative contribution rate of the first three principal components reached 87.22%. The first principal component was mainly related to germination potential, germination rate and germination index. The second principal component was mainly related to fresh bud weight and root weight. The third principal component was mainly related to root and bud length. The cluster analysis of the comprehensive score of cold tolerance of the millet resources showed that Jingtai pimple red and other 22 varieties cultivars were highly cold tolerant, Zhangchuan sesame millet and other 138 varieties cultivars were cold tolerant, Zada millet and other 64 varieties cultivars were cold sensitive, and PAN 21 and other 16 varieties cultivars were highly cold sensitive. |
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