| Effects of drought and salt stresses on leaf wax accumulation and cloning of wax alkane synthesis genesin sunflower (Helianthus annuus L.) |
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| DOI：10.7606/j.issn.1000-7601.2022.06.06 |
| Key Words: sunflower alkane gene expression drought stress |
| Author Name | Affiliation | | WEI Zhengyang | College of Agriculture, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China | | AN Peipei | College of Agriculture, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China | | WU Hongqi | College of Agriculture, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China | | LIU Le | College of Agriculture, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China | | XIAO Enshi | College of Agriculture, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China | | JING Bing | College of Agriculture, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China | | WANG Zhonghua | College of Agriculture, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China |
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| Abstract: |
| Cuticular wax plays an important role in plant stress response. To study the effects of drought and salt stresses on wax accumulation of sunflower leaves, three\|week\|old sunflower plants were separately treated with drought and salt stresses. After seven days,leaf wax was extracted and analyzed by using gas chromatography\|mass spectrometry. The results showed that the leaf wax of sunflower seedlings was mainly composed of primary alcohols, alkanes and fatty acids, which accounting for 79%, 10% and 9% of the total wax content, respectively. Drought and salt stresses did not alter the composition of leaf wax in sunflower seedlings, but resulted in a significant increase in the total wax content by 8.8% and 8.5% respectively compared to the control. Among all wax components, the content of alkanes was the most influenced and increased by 62.5% and 47.0% compared to the control after drought and salt stresses, respectively. This indicated that alkanes in sunflower seedling leaves were more sensitive to drought and salt stresses than other wax components. To further study the genes involved in wax alkane formation in sunflower, a homology search was performed in the sunflower genome, and six differentially expressed candidate genes were obtained, in which HaCER1-1 and HaCER3-1 with higher expression levels in leaves were cloned. The sequencing results showed that the coding regions of HaCER1-1 and HaCER3-1 were 1 869 bp and 1 674 bp in length, encoding proteins of 622 and 557 amino acids, respectively. The expression levels of HaCER3-1 under the treatments of sodium chloride solution and PEG6000 solution were further analyzed by qRT-PCR technology. The results showed that the expression levels of HaCER3-1 in leaves were up\|regulated by 11 times and 3.5 times at 12 h after treatments, indicating that the expression of HaCER3-1 was induced by drought and salt stress. This study provides some molecular foundation for dissecting the mechanisms of sunflower wax in response to osmotic stress and alkane biosynthesis. |
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