| Effects of long\|term combination of organic and inorganic fertilizer on bacterial community and antibiotic resistance genes in cinnamon soil |
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| DOI：10.7606/j.issn.1000-7601.2022.06.18 |
| Key Words: combination of organic and inorganic fertilizer cinnamon soil metagenomic bacterial community antibiotic resistance gene |
| Author Name | Affiliation | | LIU Zhiping | Institute of Resources and Environment, Shanxi Agricultural University, State Key Laboratory of Sustainable Dryland Agriculture（in preparation）, Taiyuan, Shanxi 030031, China | | XIE Wenyan | Institute of Resources and Environment, Shanxi Agricultural University, State Key Laboratory of Sustainable Dryland Agriculture（in preparation）, Taiyuan, Shanxi 030031, China | | YANG Zhenxing | Institute of Resources and Environment, Shanxi Agricultural University, State Key Laboratory of Sustainable Dryland Agriculture（in preparation）, Taiyuan, Shanxi 030031, China | | HU Xuechun | Institute of Resources and Environment, Shanxi Agricultural University, State Key Laboratory of Sustainable Dryland Agriculture（in preparation）, Taiyuan, Shanxi 030031, China | | MA Xiaonan | Institute of Resources and Environment, Shanxi Agricultural University, State Key Laboratory of Sustainable Dryland Agriculture（in preparation）, Taiyuan, Shanxi 030031, China | | ZHOU Huaiping | Institute of Resources and Environment, Shanxi Agricultural University, State Key Laboratory of Sustainable Dryland Agriculture（in preparation）, Taiyuan, Shanxi 030031, China |
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| Abstract: |
| To explore the effects of long\|term organic and inorganic fertilizer combined application on soil bacterial and antibiotic resistance gene diversity, 0~20 cm layer soil of five organic and inorganic fertilizer combined application treatments (N0P0M0, N2P1M1，N4P2M2, N3P2M3 and N0P0M6) were collected based on the long\|term location test of cinnamon soil in Shouyang, Shanxi Province. Metagenomic sequencing technology was applied to study the difference of bacterial community and antibiotic resistance genes in cinnamon soil under combined organic/inorganic fertilizer conditions. Results showed that the diversity of bacteria and antibiotic resistance genes in soil was significantly changed by long\|term organic/inorganic fertilizer combination. The dominant bacteria phyla were Proteobacteria, Acidobacteria, Actinobacteria, Gemmatimonadetes and Chloroflexi. There were 256 soil common resistance gene subtypes in different treatments. Soil specific antibiotic resistance gene subtypes increased at first and then decreased with the increase of fertilizer application amount, reaching the highest in N3P2M3 treatment. The antibiotic resistance genes with high abundance in soil were macrolides, multidrugs, vancomycin, tetracycline and chloramphenicol. Among them, the resistance gene subtypes with the highest content were MacB, BcrA, MexW, MexF and pbp1a. Pearson correlation analysis showed that soil physical and chemical properties directly or indirectly affected the composition and variation of bacterial community and ARGs. Redundancy analysis further showed that the changes of antibiotic resistance genes were affected by bacterial community structure. Antibiotic resistance genes have become potential pollutants threatening human health. Livestock and poultry manure should be fully decomposed and combined with chemical fertilizer in agricultural production to ensure ecological environment safety. |
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