| Development and application of SSR molecular markers based on transcriptome sequencing of adzuki bean |
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| DOI：10.7606/j.issn.1000-7601.2023.01.02 |
| Key Words: adzuki bean transcriptome SSR molecular markers |
| Author Name | Affiliation | | XU Xiaodan | Heilongjiang Provincial Key Laboratory of Crop\|Pest Interaction Biology and Ecological Control, National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | LENG Miao | Heilongjiang Provincial Key Laboratory of Crop\|Pest Interaction Biology and Ecological Control, National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | ZHANG Mingyuan | Heilongjiang Provincial Key Laboratory of Crop\|Pest Interaction Biology and Ecological Control, National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | KE Xiwang | Heilongjiang Provincial Key Laboratory of Crop\|Pest Interaction Biology and Ecological Control, National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | YIN Lihua | Heilongjiang Provincial Key Laboratory of Crop\|Pest Interaction Biology and Ecological Control, National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | ZUO Yuhu | Heilongjiang Provincial Key Laboratory of Crop\|Pest Interaction Biology and Ecological Control, National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China |
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| Abstract: |
| To develop SSR (Simple Sequence Repeats) molecular markers for adzuki bean, MISA and Primer 3 were used to search Unigenes deriving from transcriptome sequencing of adzuki bean QH1 in 2019-2020, and 3 045 SSR markers were obtained. By analyzing the characteristics of repeated motifs of markers, it was found that mononucleotide, dinucleotide and trinucleotide were the main motifs, accounting for 41.2%, 26.4% and 25.6% of total markers, respectively. A physical map containing of 1 505 SSR markers was constructed using markers that was anchored to the genome of adzuki bean and successfully designed with primers. From the above 1 505 SSR markers, 132 SSR markers distributed on 11 chromosomes of adzuki bean were randomly selected for validation. 118 markers were effectively amplified with the efficiency of 89.4%. Six polymorphic markers were obtained through polymorphic marker screening, and the clustering analysis of 36 adzuki bean cultivars using these six markers revealed that the test materials were classified into four groups. This indicated that the developed SSR molecular markers were able to analyze the genetic background differences of adzuki bean and provide usable molecular markers for germplasm identification, genetic diversity analysis and gene mapping for good traits. |
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