| Drought resistance identification and drought resistance index selection of Salvia miltiorrhiza during germination |
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| DOI：10.7606/j.issn.1000-7601.2023.01.03 |
| Key Words: Salvia miltiorrhiza drought resistance identification germination period osmotic stress drought resistance index screening |
| Author Name | Affiliation | | ZHANG Hongrui | College of Agriculture, Henan Agricultural University, Zhengzhou, Henan 450046, China | | ZHANG Lixin | College of Agriculture, Henan Agricultural University, Zhengzhou, Henan 450046, China | | LI Menghe | College of Agriculture, Henan Agricultural University, Zhengzhou, Henan 450046, China
Xinxiang Academy of Agricultural Sciences, Xinxiang, Henan 453000, China | | HE Liwei | Henan Academy of Agricultural Sciences, Changyuan Branch Changyuan, Henan 453400, China | | YANG Dandan | College of Agriculture, Henan Agricultural University, Zhengzhou, Henan 450046, China | | GAO Zhiming | College of Agriculture, Henan Agricultural University, Zhengzhou, Henan 450046, China |
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| Abstract: |
| To compare the drought resistance of different Salvia miltiorrhiza cultivar types and select drought\|resistant varieties, five Salvia miltirrohiza cultivar types were treated with different concentrations (0%, 3%, 6%, 9%, 12%) of PEG-6000 at germination stage to simulate drought stress. The germination potential, germination rate, cotyledon length, cotyledon width, radicle length and radicle length were measured. Principal component analysis and membership function analysis were used for identification of drought resistance and selection of drought resistance indexes at the germination stage of Salvia miltiorrhiza. The results showed that high concentration of PEG had an obvious inhibitory effect on seed germination, and low concentration (3% PEG-6000) treatment had a promoting effect on the growth and development of Salvia miltiorrhiza seeds. Among the five cultivation types of Salvia miltiorrhiza, Yu dan shen VD had the highest germination rate (0% PEG-6000) and germination potential (3% PEG-6000), which were 56.50% and 55.50%, respectively. Yu dan shen VC, Yu dan shen VD and Yu dan shen VE had the lowest germination rate under 12% PEG-6000, which was only 0%. With the increase of PEG stress, the germination potential, germination rate, germ length, radicle length, cotyledon length, cotyledon width, ten bud weight, germination index and vigor index showed a trend of first increasing and then decreasing. Principal component analysis was carried out for each indicator. The first two principal components summarized 82.635% of the data information of nine indicators. Factor analysis was carried out for the relative values of eight comprehensive indicators with large feature vectors in the first two principal components. The 9% PEG-6000 concentration was a more suitable one for drought resistance identification. According to the comprehensive membership function value, the order of drought resistance of five tested materials was obtained: Yu Danshen VE>Yu Danshen VC>Yu Danshen VB>Yu Danshen VD>Yu Danshen VA. The comprehensive membership function values were 0.071, 0.068, 0.054, 0.043, 0.028. Yu dan shen VE had strong drought resistance. Yu dan shen VC had the second strongest drought resistance. Yu dan shen VD and Yu dan shen VA had weaker drought resistance. Cotyledon length, cotyledon width, vitality index, ten bud weight and germination index should be used as drought resistance identification indexes. |
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