| Optimization of bio\|organic fertilizer production by Bacillus subtilis CY1 solid\|state fermentation |
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| DOI：10.7606/j.issn.1000-7601.2023.01.04 |
| Key Words: Bacillus subtilis solid state fermentation bio\|organic fertilizer response surface analysis |
| Author Name | Affiliation | | LI Bintao | College of Natural Resources and Environment, Northwest A&F University, Yangling, Shannxi 712100, China | | WEI Xiaomin | College of Natural Resources and Environment, Northwest A&F University, Yangling, Shannxi 712100, China | | LAI Hangxian | College of Natural Resources and Environment, Northwest A&F University, Yangling, Shannxi 712100, China | | LI Yulong | College of Natural Resources and Environment, Northwest A&F University, Yangling, Shannxi 712100, China | | WANG Xudong | College of Natural Resources and Environment, Northwest A&F University, Yangling, Shannxi 712100, China |
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| Abstract: |
| Bacillus subtilis CY1 is a plant broad\|spectrum rhizosphere bacterium. To promote the resource utilization of agricultural waste and improve the propagation capacity of Bacillus subtilis CY1 in the production of bio\|organic fertilizer by solid fermentation, this study developed a new bio\|organic fertilizer by solid fermentation using pig manure compost as raw material and bran, soybean meal and corn meal as auxiliary materials. The optimal ratio of raw materials and excipients and inoculation amount were determined with the number of viable bacteria as reference index. Plackett\|burman experiment design was used to evaluate the effects of eight factors on fermentation, and three factors — initial pH, fermentation temperature and water content with significant effects were selected. The steepest climbing test was used to approximate the maximum response area, and response surface analysis was used to determine the optimal fermentation parameters. The results showed that the solid\|state fermentation conditions were as follows: initial pH 7.51, KH2PO4 content 0.75%, MgSO4 content 0.15%, MnSO4 content 0.15%, fermentation temperature 36.10℃, glucose content 6.00%, (NH4)2SO4 content 0.75%, water content 26.33%. At this time, the number of viable bacteria in solid fermentation products reached the maximum, reaching 4.57×1010 CFU·g-1. This study provides a new way to produce low\|cost and high\|quality bio\|organic fertilizer by using agricultural waste in appropriate raw material ratio and technological conditions. |
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