| Effects of long\|term application of green manure on soil microorganisms in wheat maize intercropping |
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| DOI：10.7606/j.issn.1000-7601.2023.01.05 |
| Key Words: fertilization soil microorganisms long\|term positioning test community diversity |
| Author Name | Affiliation | | XU Xiaohu | Gansu Agricultural University, Institute of Soil Fertilizer and Water Saving Agriculture, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu 730070, China | | CHE Zongxian | Gansu Agricultural University, Institute of Soil Fertilizer and Water Saving Agriculture, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu 730070, China
Gansu Cultivated Land Conservation and Agricultural Environment Science Observation Experimental station, Ministry of agriculture, Wuwei, Gansu 733017, China | | ZHAO Xu | Gansu Agricultural University, Institute of Soil Fertilizer and Water Saving Agriculture, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu 730070, China | | CUI Heng | Gansu Agricultural University, Institute of Soil Fertilizer and Water Saving Agriculture, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu 730070, China
Gansu Cultivated Land Conservation and Agricultural Environment Science Observation Experimental station, Ministry of agriculture, Wuwei, Gansu 733017, China | | ZHANG Jiudong | Gansu Agricultural University, Institute of Soil Fertilizer and Water Saving Agriculture, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu 730070, China
Gansu Cultivated Land Conservation and Agricultural Environment Science Observation Experimental station, Ministry of agriculture, Wuwei, Gansu 733017, China |
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| Abstract: |
| Using Biolog microplate method and high\|throughput sequencing technology, we examined the differences of soil microbial utilization of different carbon sources and the changes of soil microbial community at the phylum and genus levels under wheat\|corn intercropping conditions with different long\|term fertilization treatments and investigated the effects of long\|term green manure application on soil microbial community diversity and metabolic functions. The results showed that long\|term application of green manure significantly increased soil organic matter, fast\|acting phosphorus and fast\|acting potassium content, reduced soil pH, and enhanced the metabolic capacity of soil microbial carbon sources. Under different fertilization conditions, Actinobacteria and Proteobacteria were the dominant soil bacterial groups, and the highest abundance of Actinobacteria was 29.94% in the green manure treatment and the lowest was 22.95% in the farmyard manure treatment; the highest abundance of Proteobacteria was 22.32% in the straw return treatment and the lowest was 18.85% in the control treatment. The relative abundance of Acidobacteriota, Chloroflexi and Firmicutes was higher in each treatment, ranging from 13.39%~19.71%, 10.85%~14.56% and 3.54%~12.98%, respectively. Long\|term application of green manure effectively increased the abundance of soil Actinobacteria, Firmicutes, Bacillus spp. and Pseudarthrobacter spp. and decreased the abundance of Acidobacteriota and increased the number of soil microbial populations. In conclusion, long\|term application of green manure not only improved soil fertility, but also enhanced the ability of soil to resist soil\|borne diseases. |
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