| Effects of exogenous SA on antioxidant enzyme activities and endogenous SA content in wine grapevine seedlings under low temperature stress |
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| DOI：10.7606/j.issn.1000-7601.2023.01.10 |
| Key Words: wine grapes low temperature salicylic acid antioxidant enzymes seedlings |
| Author Name | Affiliation | | WANG Wangtian | Gansu Provincial Key Laboratory of Aridland Crop Science, College of Life Science and Technology, Gansu Agricultural University，Lanzhou, Gansu 730070，China | | LI Bin | Institute of Biology, Gansu Academy of Sciences，Lanzhou, Gansu 730099， China | | ZHAO Junpeng | Administration of Natural Resources of Zhuanglang County, Pingliang, Gansu 744600, China | | WANG Baoqiang | Gansu Provincial Key Laboratory of Aridland Crop Science, College of Life Science and Technology, Gansu Agricultural University，Lanzhou, Gansu 730070，China | | ZHANG Rui | College of Water Conservancy and Hydropower Engineering, Gansu Agricultural University, Lanzhou, Gansu 730070，China |
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| Abstract: |
| Spring inversions in north China limit the extent of grape planting and severely affect the yield and quality of grapes. The effects of low temperature stress on the degree of membrane oxidation, antioxidant enzyme activities and endogenous SA content of grape seedlings were investigated using indoor simulated low temperature treatments. The results showed that MDA content of grape seedling leaves increased significantly by 135.64% and 68.38% at 4℃ and 12℃, respectively, compared to 25℃, and the activities of protective enzymes SOD, POD, CAT and endogenous SA content increased significantly 133.05%, 53.33%, 129.17%, 46.22%, 59.65%, 26.67%, 29.17%, 24.65%. The antioxidant enzyme activities and endogenous SA content were increased significantly at 4℃ compared to 12℃, SOD, POD, CAT activities and endogenous SA contents increased by 45.97%, 21.05%, 77.42% and 17.31%, respectively. The antioxidant enzymes SOD, POD, CAT activities and endogenous SA content of low temperature treated seedlings leaves did not decrease significantly after restoration of normothermic growth and had a sustained effect on the repair of grape seedling tissue, and the antioxidant enzymes SOD, POD and CAT activities of the seedlings treated at 4℃ were 1.29, 1.09 and 1.30 times higher than those of the 12℃ treatment. The correlation and stepwise regression analysis found that endogenous SA was positively correlated with MDA content (R2=0.648), SOD (R2=0.847), POD (R2=0.436), and CAT (R2=0.584) activities under low temperature. There was a linear regression relationship between the activities of SOD, CAT and POD, and the content of endogenous SA. Antioxidant enzyme activities were regulated by both endogenous SA and low temperature signal. The antioxidant enzymes and endogenous SA played a sustained role in mitigating the damage of seedlings by low temperature stress. Exogenous SA and low temperature stress induced the accumulation of endogenous SA, which significantly increased the activities of SOD, POD, and CAT and improved the tolerance of grapes to low temperature stress. |
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