| Effects of Trichoderma on physiological characteristics of sunflower seedlings and control efficacy against Sclerotinia sclerotiorum |
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| DOI：10.7606/j.issn.1000-7601.2023.01.20 |
| Key Words: Trichoderma sunflower Sclerotinia sclerotiorun physiological characteristics control efficacy |
| Author Name | Affiliation | | LIAN Hua | College of Horticulture and Landscape Architecture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | CHEN Yurong | College of Horticulture and Landscape Architecture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | LI Mei | Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China | | LI Runzhe | College of Horticulture and Landscape Architecture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | ZHANG Ting | College of Horticulture and Landscape Architecture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | MA Guangshu | College of Horticulture and Landscape Architecture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China |
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| Abstract: |
| T. afroharzianum 838 and T. asperellum 573 with good control effect against sunflower Sclerotinia sclerotiorun were selected for the study. Different strains application methods on seedling growth, physiological characteristics and control effect against Sclerotinia sclerotiorun of sunflower through pot culture were evaluated from May to August 2021. The results showed that application methods of T. afroharzianum 838 and T. asperellum 573 effectively improved the control effect of Sclerotinia sclerotiorum of sunflower. T1 of T. afroharzianum 838（mixing soil with 100 ml· pot-1 Trichoderma suspension 5 days before sowing+root irrigation with 100 ml pot-1 Trichoderma suspension 2 days before inoculation of Sclerotinia sclerotiorum） was the most effective one with disease control efficacy of 98.58%. Different application methods of T. afroharzianum 838 and T. asperellum 573 all improved the plant height, stem diameter, whole plant fresh weight and root shoot ratio of sunflower seedlings compared with CK1 (only using sunflower Sclerotinia sclerotiorum to irrigate roots without Trichoderma suspension). T1 treatment of T. afroharzianum 838 had the best promoting effect on the morphogenesis and material accumulation of sunflower seedlings. Compared with CK1, plant height, stem diameter, whole plant fresh weight and root shoot ratio of sunflower seedlings increased by 58.82%, 177.42%, 595.17% and 41.67% respectively. Different application methods of T. afroharzianum 838 and T. asperellum 573 all increased the contents of soluble sugar, soluble protein, proline and chlorophyll in the leaves of sunflower seedlings, and reduced the content of MDA and cell membrane relative permeability in the leaves. T1 treatment of T. afroharzianum 838 had the best effect on promoting the physiological characteristics of sunflower seedlings. Compared with CK1, the contents of soluble sugar, soluble protein, proline and chlorophyll in the leaves of sunflower seedlings increased by 179.91%, 132.10%, 98.68% and 129.27% respectively, while the content of MDA and cell membrane relative permeability decreased by 49.44% and 53.22% respectively at 35 days after sowing. The results showed that T. afroharzianum 838 and T. asperellum 573 in this study increased the physiological characteristics, promoted the morphogenesis and material accumulation of sunflower seedlings and improved the control effect of sunflower Sclerotinia sclerotiorun. |
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